BAP1 Antikörper (AA 36-66)

Produktnummer ABIN388948

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch BAP1 in WB, IF, IHC (p) und FACS. Er zeigt eine Reaktivität gegenüber Human, Maus und Ratte und wurde in 1 Publikation erwähnt.

Kurzübersicht für BAP1 Antikörper (AA 36-66) (ABIN388948)

Target: BAP1 (BRCA1 Associated Protein-1 (Ubiquitin Carboxy-terminal Hydrolase) (BAP1))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser BAP1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS)

Klon: RB4559-4560

Produktdetails anti-BAP1 Antikörper

Bindungsspezifität: AA 36-66

Homologie: B, Zf

Aufreinigung: This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen: This BAP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 36-66 amino acids of human BAP1.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: IF: 1:25. WB: 1:2000. WB: 1:1000. IHC-P: 1:100. IHC-P: 1:100. FC: 1:25. IHC: 1:250

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C.

Haltbarkeit: 6 months

Referenzen für anti-BAP1 Antikörper (ABIN388948)

Abdel-Rahman, Pilarski, Cebulla, Massengill, Christopher, Boru, Hovland, Davidorf: "Germline BAP1 mutation predisposes to uveal melanoma, lung adenocarcinoma, meningioma, and other cancers." in: Journal of medical genetics, Vol. 48, Issue 12, pp. 856-9, (2011) (PubMed).

Details zu BAP1

Target: BAP1 (BRCA1 Associated Protein-1 (Ubiquitin Carboxy-terminal Hydrolase) (BAP1))

Andere Bezeichnung: BAP1

Hintergrund: 'BRCA1-associated protein-1,' or BAP1 interacts with the RING finger domain of BRCA1. The N-terminal 240 amino acids of the predicted 729-amino acid human protein show homology to ubiquitin C-terminal hydrolases (UCHs), thiol proteases that catalyze proteolytic processing of ubiquitin. In addition, BAP1 contains an acidic region, a highly charged C-terminal region, and 2 putative nuclear localization signals.. BAP1 and BRCA1 associate in vivo and have overlapping subnuclear localization patterns.1 BAP1 enhances BRCA1-mediated inhibition of breast cancer cell growth. Northern blot analysis indicates that BAP1 is expressed as a 4-kb mRNA in all human tissues tested, with A 4.8-kb transcript expressed exclusively in testis. Northern blot analysis and in situ hybridization reveal that BAP1 and BRCA1 are coexpressed during murine breast development and remodeling. The BAP1 gene has been mapped to 3p21.3, a region of loss of heterozygosity for breast cancer as well as frequently deleted in lung carcinomas.1 Intragenic homozygous rearrangements and deletions of BAP1 appear in lung carcinoma cell lines. It has been postulated that BAP1 is a tumor suppressor gene that functions in the BRCA1 growth control pathway.1

Molekulargewicht: 80362

NCBI Accession: NP_004647

UniProt: Q92560