His Tag Antikörper (Biotin)

Produktnummer ABIN387702

Zitiert in 2+ Publikationen. Der Biotin-konjugierte Maus Monoklonal anti-His Tag Antikörper (Klon 6G2A9) (ABIN387702) detektiert spezifisch His Tag in WB und ELISA.

Kurzübersicht für His Tag Antikörper (Biotin) (ABIN387702)

Target: His Tag

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser His Tag Antikörper ist konjugiert mit Biotin

Applikation: Western Blotting (WB), ELISA

Klon: 6G2A9

Produktdetails anti-His Tag Antikörper (Biotin)

Sequenz: HHHHHH

Spezifität: THE^TM His Tag Antibody [Biotin], mAb, Mouse specificly recognizes C-terminal, N-terminal, and internal His tagged fusion proteins.

Produktmerkmale: The antibody is suitable for detecting fusion proteins that contain a His-tag and it is supplied as a 100 µL aliquot at a concentration of 1 mg/mL.

Aufreinigung: Protein A affinity column

Immunogen: A synthetic peptide (HHHHHH) coupled - KLH

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Working concentrations for specific applications should be determined by the investigator. Appropriate concentrations will be affected by several factors, including secondary antibody affinity, antigen concentration, sensitivity of detection method, temperature, and length of incubations, etc. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.02-0.2 µg/mL
Western blot: 0.1-1.0 µg/mL
Other applications: user-optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Konzentration: 0.5 mg/mL

Buffer: PBS, pH 7.4, containing 1 % BSA and 0.01 % Sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handhabung: Avoid repeated freezing and thawing cycles.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Referenzen für anti-His Tag Antikörper (Biotin) (ABIN387702)

Wegman, Geuze, van der Helm, Cumhur Öner, Dhert, Alblas: "Gene delivery of bone morphogenetic protein-2 plasmid DNA promotes bone formation in a large animal model." in: Journal of tissue engineering and regenerative medicine, Vol. 8, Issue 10, pp. 763-70, (2014) (PubMed).

Ota, Doyle-Cooper, Cooper, Huber, Falkowska, Doores, Hangartner, Le, Sok, Jardine, Lifson, Wu, Mascola, Poignard, Binley, Chakrabarti, Schief, Wyatt, Burton, Nemazee: "Anti-HIV B Cell lines as candidate vaccine biosensors." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 189, Issue 10, pp. 4816-24, (2012) (PubMed).

Details zu His Tag

Target: His Tag

Substanzklasse: Tag