EGR2 Antikörper (Internal Region)

Produktnummer ABIN375194

Der Ziege Polyklonal anti-EGR2 Antikörper wird verwendet zum Nachweis von EGR2 in Proben von Human, Maus, Ratte und Hund. Er wurde validiert für WB, IHC (p) und EIA.

Kurzübersicht für EGR2 Antikörper (Internal Region) (ABIN375194)

Target: EGR2 (Early Growth Response 2 (EGR2))

Reaktivität: Human, Maus, Ratte, Hund

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser EGR2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Produktdetails anti-EGR2 Antikörper

Bindungsspezifität: Internal Region

Sequenz: HGTAGPDRKP FPC

Spezifität: This antibody recognizes EGR2

Kreuzreaktivität (Details): Species reactivity (expected):Mouse, Rat and Canine.
Species reactivity (tested):Human.

Aufreinigung: Ammonium sulphate precipitation followed by antigen Affinity Chromatography using the immunizing peptide.

Immunogen: Peptide with sequence from the internal region of the protein sequence according to NP_000390.2. Genename: EGR2

Anwendungsinformationen

Applikationshinweise: Peptide ELISA: Detection Limit: 1/64000. Western blot: 0.03-0.1 μg/mL. Approx 55 kDa band observed in lysates of cell line HepG2(calculated MW of 50.5 kDa according to NP_000390.2). Immunohistochemistry: 3-5 μg/mL. In paraffin embedded Human Colon shows nuclearstaining of absorptive cells.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu EGR2

Target: EGR2 (Early Growth Response 2 (EGR2))

Andere Bezeichnung: EGR2

Hintergrund: ERG2 is a sequence-specific transcription factor, that binds to two specific DNA sites located in the promoter region of HOXA4. Defects in EGR2 are a cause of congenital hypomyelination neuropathy (CHN), which can be inherited in either an autosomal dominant or recessive manner. Recessive CHN can present as Charcot-Marie-Tooth disease type 4E, type 1D, or Dejerine-Sottas syndrome (DSS), a group of neuropathies associated with decreased nerve conduction velocities.Synonyms: AT591, EGR-2, Early growth response protein 2, KROX20, Krox 20, Zinc finger protein Krox-20

Gen-ID: 1959

NCBI Accession: NP_000390

UniProt: P11161