SULF2 Antikörper (Internal Region)

Produktnummer ABIN375090

Produktdetails anti-SULF2 Antikörper

Target: SULF2 (Sulfatase 2 (SULF2))

Bindungsspezifität: Internal Region

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser SULF2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Spezifität: This antibody is expected to recognize both reported isoforms (NP_061325.1 and NP_940998.1).

Aufreinigung: Affinity Chromatography

Immunogen: Peptide with sequence C-DGRVYHVGLGDAAQPR, from the internal region of the protein sequence

Anwendungsinformationen

Applikationshinweise: Peptide ELISA: 1/8000. Western Blot: 0.3 - 1 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3 containing 0.02 % Sodium Azide and 0.5 % BSA

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu SULF2

Target: SULF2 (Sulfatase 2 (SULF2))

Andere Bezeichnung: SULF2 (SULF2 Produkte)

Hintergrund: Sulf-2 exhibits arylsulfatase activity and highly specific endoglucosamine-6-sulfatase activity. It can remove sulfate from the C-6 position of glucosamine within specific subregions of intact heparin. Mammalian Sulfs are endoproteolytically processed and secreted into the extracellular space of transfected cells, where they exhibit both arylsulfatase activity and highly specific endoglucosamine-6-sulfatase activity against intact heparin. Though the molecular weight of full length human Sulf2 is 100455 Da (870 amino acids), the endogenous Sulf2 is found by western blot to be present at molecular weights of 24.4, 33.7 and 51-60 kDa. Higher molecular weight species may be found depending on tissue and/or cell type. Analysis of human tumor tissue and tumor cell lines suggests that HSulf-1 is misregulated in cancers. HSulf-1 is found in a variety of normal tissues but is down-regulated in tumor cell lines originating from ovarian, breast, pancreatic, renal, and hepatocellular carcinoma. Tumors formed by cells expressing Sulf demonstrate enhanced extracellular matrix deposition. The 6-O-sulfation of heparan sulfate of myeloma tumor cells may be a critical factor in determiningand regulating the in vivo growth of this cancer.Synonyms: Extracellular sulfatase Sulf-2, KIAA1247

Gen-ID: 55959, 9606

UniProt: Q8IWU5