FTL Antikörper (C-Term, Light Chain)

Produktnummer ABIN375068

Der Ziege Polyklonal Anti-FTL-Antikörper wurde für WB, IHC (p) und EIA validiert. Er ist geeignet, FTL in Proben von Human, Maus, Ratte, Rind (Kuh) und Hund zu detektieren. Es ist 1 Publikation verfügbar.

Kurzübersicht für FTL Antikörper (C-Term, Light Chain) (ABIN375068)

Target: FTL (Ferritin, Light Polypeptide (FTL))

Reaktivität: Human, Maus, Ratte, Rind (Kuh), Hund

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser FTL Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Produktdetails anti-FTL Antikörper

Bindungsspezifität: C-Term, Light Chain

Sequenz: C-GEYLFERLTL KHD

Spezifität: This antibody recognizes FTL.

Kreuzreaktivität (Details): Species reactivity (expected):Mouse, Rat, Canine (Dog), Bovine.
Species reactivity (tested):Human.

Aufreinigung: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide

Immunogen: Synthetic peptide (C-GEYLFERLTLKHD) from the C-Terminus of Human FTL (NP_000137.2). Genename: FTL

Anwendungsinformationen

Applikationshinweise: Peptide ELISA: 1/4000. Western Blot: 0.1 - 0.3 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Referenzen für anti-FTL Antikörper (ABIN375068)

Sammarco, Ditch, Banerjee, Grabczyk: "Ferritin L and H subunits are differentially regulated on a post-transcriptional level." in: The Journal of biological chemistry, Vol. 283, Issue 8, pp. 4578-87, (2008) (PubMed).

Details zu FTL

Target: FTL (Ferritin, Light Polypeptide (FTL))

Andere Bezeichnung: Ferritin Light Chain (FTL)

Hintergrund: Ferritin is a ubiquitous and highly conserved protein which plays a major role in iron homeostasis by sequestering and storing iron in a non-toxic and soluble form. It forms a holoenzyme of ~450 kDa, consisting of 24 subunits of two types, H (heavy, 21 kDa) and L (light, 19 kDa), and is capable of storing up to 4,500 atoms of ferric iron. Depending on the tissue type and physiological status of the cell, the ratio of H to L subunits in ferritin can vary widely. Ferritin is found in the liver, spleen, kidney and heart, with smaller amounts being found in blood. Serum ferritin levels serve as an indicator of the amount of iron stored in the body. Serum ferritin is the most sensitive test for anaemia, and is also used as a marker for restless leg syndrome, hemochromatosis and porphyria. As ferritin is an acute-phase reactant, it is often elevated during infection. Defects in ferritin proteins are associated with several neurodegenerative diseases.Synonyms: Ferritin L subunit

Molekulargewicht: 20.0 kDa (NP_000137.2).

Gen-ID: 2512

NCBI Accession: NP_000137

UniProt: P02792

Pathways: Transition Metal Ion Homeostasis