EGLN3 Antikörper (Internal Region)

Produktnummer ABIN374996

Der Ziege Polyklonal anti-EGLN3 Antikörper wird verwendet zum Nachweis von EGLN3 in Proben von Human, Maus, Ratte, Rind (Kuh) und Hund. Er wurde validiert für WB und EIA.

Kurzübersicht für EGLN3 Antikörper (Internal Region) (ABIN374996)

Target: EGLN3 (Egl-9 Family Hypoxia Inducible Factor 3 (EGLN3))

Reaktivität: Human, Maus, Ratte, Rind (Kuh), Hund

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser EGLN3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Enzyme Immunoassay (EIA)

Produktdetails anti-EGLN3 Antikörper

Bindungsspezifität: Internal Region

Spezifität: This antibody recognizes EGLN3.

Kreuzreaktivität (Details): Species reactivity (expected):Mouse, Rat, Canine, Bovine.
Species reactivity (tested):Human.

Aufreinigung: Affinity chromatography

Immunogen: Peptide with sequence C-RLGKYYVKERSK, from the internal region of the protein sequence

Anwendungsinformationen

Applikationshinweise: Peptide ELISA: 1/8000. Western Blot: 0.1 - 0.3 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0,5 mg/mL

Buffer: Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu EGLN3

Target: EGLN3 (Egl-9 Family Hypoxia Inducible Factor 3 (EGLN3))

Andere Bezeichnung: EGLN3 / PHD3

Hintergrund: PHD3 catalyzes the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins, hydroxylates HIF-1 alpha at Pro-564, and also hydroxylates HIF-2 alpha. It functions as a cellular oxygen sensor and, under normoxic conditions, targets HIF through the hydroxylation for proteasomal degradation via the von Hippel-Lindau ubiquitylation complex. It may play a role in cell growth regulation in muscle cells and in apoptosis in neuronal tissue. It promotes cell death through a caspase-dependent mechanism.Synonyms: Egl nine homolog 3, HIF PH3, HIF-PH3, HIF-prolyl hydroxylase 3, HPH-1, HPH-3, Hypoxia-inducible factor prolyl hydroxylase 3, Prolyl hydroxylase domain-containing protein 3

Gen-ID: 112399

NCBI Accession: NP_071356

UniProt: Q9H6Z9

Pathways: Positive Regulation of Endopeptidase Activity