PCK1 Antikörper (C-Term, Internal Region)

Produktnummer ABIN374797

Dieser Ziege Polyklonal Antikörper detektiert spezifisch PCK1 in WB. Es zeigt Reaktivität gegenüber Proben von Human.

Kurzübersicht für PCK1 Antikörper (C-Term, Internal Region) (ABIN374797)

Target: PCK1 (phosphoenolpyruvate Carboxykinase 1 (Soluble) (PCK1))

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser PCK1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Produktdetails anti-PCK1 Antikörper

Bindungsspezifität: C-Term, Internal Region

Sequenz: C-EKEVEDIEKY LEDQ

Spezifität: This antibody detects PEP carboxykinase.

Kreuzreaktivität (Details): Species reactivity (tested):Human

Aufreinigung: Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

Immunogen: Peptide from the internal region (near the C Terminus) of the protein sequence according to NP_002582.2. Genename: PCK1

Anwendungsinformationen

Applikationshinweise: Peptide ELISA: antibody detection limit dilution 1: 16000. Western blot: 0.5-1.5 μg/mL. Approx 70 kDa band observed in Human Kidney lysates(calculated MW of 69.0 kDa according to NP_002582.2).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C.

Details zu PCK1

Target: PCK1 (phosphoenolpyruvate Carboxykinase 1 (Soluble) (PCK1))

Andere Bezeichnung: PCK1 / PEPCK1

Hintergrund: PCK1 is a main control point for the regulation of gluconeogenesis. This cytosolic enzyme, along with GTP, catalyzes the formation of phosphoenolpyruvate from oxaloacetate, with the release of carbon dioxide and GDP. The expression of the corresponding gene can be regulated by insulin, glucocorticoids, glucagon, cAMP, and diet. A mitochondrial isozyme has also been characterized.Synonyms: PEP carboxykinase, PEPCK-C, PEPCKC, Phosphoenolpyruvate carboxykinase cytosolic [GTP], Phosphoenolpyruvate carboxylase

Gen-ID: 5105

NCBI Accession: NP_002582

UniProt: P35558

Pathways: Positive Regulation of Peptide Hormone Secretion, Carbohydrate Homeostasis