TIA1 Antikörper (Internal Region)

Produktnummer ABIN374795

Der Ziege Polyklonal anti-TIA1 Antikörper wird verwendet zum Nachweis von TIA1 in Proben von Human, Maus, Ratte und Hund. Er wurde validiert für WB.

Kurzübersicht für TIA1 Antikörper (Internal Region) (ABIN374795)

Target: TIA1 (TIA1 Cytotoxic Granule-Associated RNA Binding Protein (TIA1))

Reaktivität: Human, Maus, Ratte, Hund

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser TIA1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Produktdetails anti-TIA1 Antikörper

Bindungsspezifität: Internal Region

Sequenz: C-PKSTYESNTK Q

Spezifität: This antibody detects TIA1.

Kreuzreaktivität (Details): Species reactivity (expected):Mouse, Rat, DogSpecies reactivity (tested):Human

Aufreinigung: Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

Immunogen: Peptide from the internal region of the protein sequence according to NP_071320.1, NP_071505.1 Genename: TIA1

Anwendungsinformationen

Applikationshinweise: Peptide ELISA: antibody detection limit dilution 1: 128000. Western blot: 0.1-0.3 μg/mL. Approx 40 kDa band observed in lysates of cell line Jurkat

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C.

Details zu TIA1

Target: TIA1 (TIA1 Cytotoxic Granule-Associated RNA Binding Protein (TIA1))

Andere Bezeichnung: TIA1

Hintergrund: TIA1 is a member of an RNA binding protein family and possesses nucleolytic activity against cytotoxic lymphocyte (CTL) target cells. It has been suggested that this protein may be involved in the induction of apoptosis as it preferentially recognizes poly(A) homopolymers and induces DNA fragmentation in CTL targets. The major granule-associated species is a 15- kDa protein that is thought to be derived from the carboxyl terminus of the 40- kDa product by proteolytic processing. TIA-1 (T-cell intracytoplasmic antigen) monoclonal antibody reacts with a 15 kDa cytoplasmic granule-associated protein, expressed in lymphocytes processing cytolytic potential. The expression of TIA-1 was studied in CD30+ anaplastic large cell lymphomas (ALCL), NK cell lymphomas and peripheral T-cell lymphomas and T cell lymphocytosis, B-cell lymphomas and lymphoblastic leukemia, Hodgkin's etc. Studies showed that 60 to 70 % of anaplastic large cell lymphoma reacted with TIA-1. Studies also indicate that TIA-1 reacts with most large granular lymphocytic leukemia's, hepatosplenic T-cell lymphomas, intestinal T-cell lymphomas, NK-like T-cell lymphomas, NK-cell lymphomas, nasal T/NK-cell lymphomas, subcutaneous T-cell lymphomas, pulmonary angiocentric lymphomas of T or NK phenotype. The author's concluded from TIA-1 studies that anaplastic large-cell lymphomas are cytotoxic T-or NK-cell neoplasms. All B-cell lymphomas, Hodgkin's and lymphoblastic leukemias were negative for TIA-1.Synonyms: Nucleolysin TIA-1, RNA-binding protein TIA-1, TIA1 cytotoxic granule-associated RNA binding protein, p15-TIA-1, p40-TIA-1

Gen-ID: 7072, 9606

UniProt: P31483