PPID Antikörper (Internal Region)

Produktnummer ABIN374769

Der Ziege Polyklonal anti-PPID Antikörper (ABIN374769) detektiert spezifisch PPID in WB. Dieser Antikörper reagiert spezifisch mit Proben aus Human, Maus, Ratte und Hund.

Kurzübersicht für PPID Antikörper (Internal Region) (ABIN374769)

Target: PPID (Peptidylprolyl Isomerase D (PPID))

Reaktivität: Human, Maus, Ratte, Hund

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser PPID Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Produktdetails anti-PPID Antikörper

Bindungsspezifität: Internal Region

Spezifität: This antibody reacts to PPID / CyP-40.

Kreuzreaktivität (Details): Species reactivity (expected):Mouse, Rat, Canine.
Species reactivity (tested):Human.

Aufreinigung: Affinity chromatography

Immunogen: Peptide with sequence C-DENFHYKHDREG, from the internal region of the protein sequence

Anwendungsinformationen

Applikationshinweise: Peptide ELISA: 1/64000. Western Blot: 0.1 - 0.3 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu PPID

Target: PPID (Peptidylprolyl Isomerase D (PPID))

Andere Bezeichnung: Cyclophilin-40

Hintergrund: Cyclophilin 40 is a widely expressed member of the cyclophilin type PPIase family, PPIase D subfamily. It contains 1 PPIase cyclophilin type domain and 3 TPR repeats. It catalyzes the cis trans isomerization of proline imidic peptide bonds in oligopeptides. and has a role in accelerating the folding of proteins. It is less sensitive to inhibition by cyclosporin A than CYP18. Immunophilins are a family of soluble cytosolic receptors capable of binding to one of two major immunosuppressant agents: cyclosporin A (CsA) or FK506. Proteins that bind FK506 are termed FK506 Binding Proteins (FKBPs) and those that bind cyclosporin A are called cyclophilins (CyP). Both CyP:CsA and FKBP:FK506 complexes have been shown to inhibit calcineurin, a calcium and calmodulin dependent protein phosphatase which has been implicated as an important signaling enzyme in T-cell activation, providing a possible mechanism of immunosuppression by CsA and FK506. Immunophilins function as peptidyl prolyl cis-trans-isomerases (PPIase) whose activity is inhibited by their respective immunosuppressant compounds. As PPIase's, immunophilins accelerate folding of some proteins both in vivo and in vitro by catalyzing slow steps in the initial folding and rearrangement of proline containing proteins. CyP-40, a 40 kDa protein, shares significant homology with smaller CyPA (CyP-18) and FKBP59. CyP-40 exhibits the characteristic CsA binding and isomerase activity of CyP-18, though these activities appear to be less with CyP-40 than with Cyp-18. Like FKBP59, CyP-40 has been found in progesterone receptor complexes. CyP-40 is expressed at similar levels in many tissues.Synonyms: 40 kDa peptidyl-prolyl cis-trans isomerase, CYP-40, CYP40, CYPD, Cyclophilin D, Cyclophilin-related protein, PPID, PPIase D, Rotamase D

Gen-ID: 5481, 9606

UniProt: Q08752

Pathways: Nuclear Hormone Receptor Binding