FH Antikörper (Internal Region)

Produktnummer ABIN374767

Dieser Ziege Polyklonal Antikörper detektiert spezifisch FH in WB und EIA. Es zeigt Reaktivität gegenüber Proben von Human, Maus, Ratte und Hund.

Kurzübersicht für FH Antikörper (Internal Region) (ABIN374767)

Target: FH (Fumarate Hydratase (FH))

Reaktivität: Human, Maus, Ratte, Hund

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser FH Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Enzyme Immunoassay (EIA)

Produktdetails anti-FH Antikörper

Bindungsspezifität: Internal Region

Sequenz: C-HPNDHVNKSQ SSND

Spezifität: This antibody recognizes Fumarase/FH

Kreuzreaktivität (Details): Species reactivity (expected):Mouse, Rat and Canine (Dog).
Species reactivity (tested):Human.

Aufreinigung: Immunoaffinity Chromatography

Immunogen: Peptide with sequence from the internal region of the protein sequence according to NP_000134.2 Genename: FH

Anwendungsinformationen

Applikationshinweise: Peptide ELISA: 1/64000. Western Blot: 0.01-0.03 μg/mL. Approx 48 kDa band observed in Human Kidney, Liver andLung lysates. The observed molecular weight corresponds to earlier findings withantibodies from different sources. In transfected HEK293 transiently expressing Fumarasea band of approx.58 kDa is observed. This band is not observed in the non-transfectedHEK293.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3 containing 0.02 % Sodium Azide and 0.5 % BSA

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu FH

Target: FH (Fumarate Hydratase (FH))

Andere Bezeichnung: Fumarase

Hintergrund: FH (Fumarate hydratse) catalyzes the conversion of fumarate to malate. It is a key enzyme in the tricarboxylic acid (TCA) cycle. It exists in a cytosolic form and an N terminal extended form. The N terminal extended form is targeted to the mitochondrion, where the removal of the extension generates the same form as in the cytoplasm. FH also acts as a tumor suppressor.Synonyms: HLRCC, LRCC, MCL, MCUL1, mitochondrial Fumarate hydratase

Molekulargewicht: 54.6 kDa (NP_000134.2).

Gen-ID: 2271, 9606

UniProt: P07954