SV2A Antikörper (Internal Region)

Produktnummer ABIN374729

Produktdetails anti-SV2A Antikörper

Target: SV2A (Synaptic Vesicle Glycoprotein 2A (SV2A))

Bindungsspezifität: Internal Region

Reaktivität: Human, Ratte, Maus, Hund

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser SV2A Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Spezifität: This antibody recognizes SV2A.

Kreuzreaktivität (Details): Species reactivity (expected):Mouse, Human, Canine.
Species reactivity (tested):Rat.

Aufreinigung: Affinity chromatography

Immunogen: Peptide with sequence C-TVELYPSDKRTTA, from the internal region of the protein sequence

Anwendungsinformationen

Applikationshinweise: Peptide ELISA: 1/64000. Western Blot: 0.3-1 μg/mL. Approx 80 kDa band observed in rat brain lysates (calculatedMW of 82.0 kDa according to NP_476558.1 (rat)). An additonal band of unknown identitywas also consistently observed at 20 kDa. This band was successfully blocked byincubation with the immunising peptide.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: Tris saline, 0.02 % Sodium Azide, pH 7.3 with 0.5 % BSA

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu SV2A

Target: SV2A (Synaptic Vesicle Glycoprotein 2A (SV2A))

Andere Bezeichnung: SV2A (SV2A Produkte)

Hintergrund: SV2s (Synaptic Vesicle protein 2) are integral membrane glycoproteins present in all synaptic vesicles. They have 12 transmembrane domains predicted by sequence analysis. There are three characterized isoforms, SV2A, SV2B and SV2C. SV2A is expressed ubiquitously throughout the brain. SV2B has a more restricted distribution with varying degrees of coexpression with SV2A. SV2C is more closely related to SV2A but shows a very restricted expression pattern. The highest expression levels were observed in phylogenetically old brain areas like pallidum, the midbrain and the olfactory bulb. SV2A is probably involved in the maintenance of a pool of synaptic vesicles competent for calcium-stimulated exocytosis. SV2A is a highly glycosylated synaptic vesicle protein with homology to transmembrane transporters.Synonyms: KIAA0736, Synaptic vesicle glycoprotein 2A

Gen-ID: 9900

NCBI Accession: NP_055664

UniProt: Q7L0J3