GPX4 Antikörper (C-Term, Isoform A)

Produktnummer ABIN374711

Der Ziege Polyklonal anti-GPX4 Antikörper wird verwendet zum Nachweis von GPX4 in Proben von Human, Maus, Ratte, Rind (Kuh) und Schwein. Er wurde validiert für WB, IHC (p) und EIA.

Kurzübersicht für GPX4 Antikörper (C-Term, Isoform A) (ABIN374711)

Target: GPX4 (Glutathione Peroxidase 4 (GPX4))

Reaktivität: Human, Maus, Ratte, Rind (Kuh), Schwein

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser GPX4 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Produktdetails anti-GPX4 Antikörper

Bindungsspezifität: C-Term, Isoform A

Spezifität: This antibody is expected to recognize isoform A (NP_002076.2) and isoform C (NP_001034937.1).

Kreuzreaktivität (Details): Species reactivity (expected):Mouse, Rat, Pig, Cow.
Species reactivity (tested):Human.

Aufreinigung: Affinity Chromatpography

Immunogen: Peptide with sequence (C-EEPLVIEKDLPHY) from the C-terminus of the protein sequencen (NP_002076.2, NP_001034937.1).

Anwendungsinformationen

Applikationshinweise: ELISA: 1/16000. Western Blot: 0.1 - 0.3 μg/mL. Immunohistochemistry on paraffin sections: 0.3 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu GPX4

Target: GPX4 (Glutathione Peroxidase 4 (GPX4))

Andere Bezeichnung: Glutathione Peroxidase 4 / GPX4

Hintergrund: Glutathione peroxidases (Gpxs) are ubiquitously expressed proteins which catalyze the reduction of hydrogen peroxides and organic hydroperoxides by glutathione. There are several isoforms which differ in their primary structure and localization. The classical cytosolic/mitochondrial GPx1 (cGPx) is a selenium-dependent enzyme, first of the GPx family to be discovered. GPx2, also known as gastrointestinal GPx (GI-GPx), is an intracellular enzyme expressed only at the epithelium of the gastrointestinal tract. Extracellular plasma GPx (pGPx or GPx3) is mainly expressed by the kidney from where it is released into the blood circulation. Phospholipid hydroperoxide GPx4 (PH-GPx) expressed in most tissues, can reduce many hydroperoxides including hydroperoxides integrated in membranes, hydroperoxy lipids in low density lipoprotein or thymine. All mammalian GPx family members, except for the recently described Cys containing GPx3 and epididymis-specific secretory GPx (eGPx or GPx5) isoforms, possess selenocysteine at the active site.Synonyms: GPx-4, Phospholipid hydroperoxide glutathione peroxidase mitochondrial

Gen-ID: 2879

NCBI Accession: NP_001034936

UniProt: P36969