PEPCK Antikörper (Internal Region)

Produktnummer ABIN374610

Zitiert in 2+ Publikationen. Der Ziege Polyklonal anti-PEPCK Antikörper (ABIN374610) detektiert spezifisch PEPCK in WB und EIA. Dieser Antikörper reagiert spezifisch mit Proben aus Human, Maus, Ratte, Rind (Kuh), Hund und Schwein.

Kurzübersicht für PEPCK Antikörper (Internal Region) (ABIN374610)

Target: PEPCK (phosphoenolpyruvate Carboxykinase 2 (Mitochondrial) (PEPCK))

Reaktivität: Human, Maus, Ratte, Rind (Kuh), Hund, Schwein

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser PEPCK Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Enzyme Immunoassay (EIA)

Produktdetails anti-PEPCK Antikörper

Bindungsspezifität: Internal Region

Sequenz: KPWKPGDKEP CAH

Spezifität: This antibody recognizes PCK2. This antibody is expected to recognise isoform 1 only, no crossreactivity with PCK1.

Kreuzreaktivität (Details): Species reactivity (expected):Canine, Bovine, Porcine.
Species reactivity (tested):Human, Mouse, Rat, Guinea Pig.

Aufreinigung: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide.

Immunogen: Peptide with sequence from the internal region of the protein sequence according to NP_004554.2. Genename: PCK2

Anwendungsinformationen

Applikationshinweise: Peptide ELISA: Limit Dilution: 1/1000. Western blot: 0.1-0.3 μg/mL. Approx 70 kDa band observed in Human Liver lysates and inlysates of cell line HepG2 (calculated MW of 70.0 kDa according to NP_004554.2).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Referenzen für anti-PEPCK Antikörper (ABIN374610)

Méndez-Lucas, Duarte, Sunny, Satapati, He, Fu, Bermúdez, Burgess, Perales: "PEPCK-M expression in mouse liver potentiates, not replaces, PEPCK-C mediated gluconeogenesis." in: Journal of hepatology, Vol. 59, Issue 1, pp. 105-13, (2013) (PubMed).

Idnurm: "Light sensing in Aspergillus fumigatus highlights the case for establishing new models for fungal photobiology." in: mBio, Vol. 4, Issue 3, pp. e00260-13, (2013) (PubMed).

Details zu PEPCK

Target: PEPCK (phosphoenolpyruvate Carboxykinase 2 (Mitochondrial) (PEPCK))

Andere Bezeichnung: PCK2

Hintergrund: PCK2 is a a member of the phosphoenolpyruvate carboxykinase (GTP) family. The protein is a mitochondrial enzyme that catalyzes the conversion of oxaloacetate to phosphoenolpyruvate in the presence of GTP. A cytosolic form encoded by a different gene has also been characterized and is the key enzyme of gluconeogenesis in the liver. The encoded protein may serve a similar function, although it is constitutively expressed and not modulated by hormones such as glucagon and insulin that regulate the cytosolic form. Alternatively spliced transcript variants have been described.Synonyms: EC=4.1.1.32, PCK-2, PEPCK-M, PEPCK2, Phosphoenolpyruvate carboxykinase, Phosphoenolpyruvate carboxylase

Gen-ID: 5106

NCBI Accession: NP_001018083

UniProt: Q16822

Pathways: Positive Regulation of Peptide Hormone Secretion, Carbohydrate Homeostasis