DAZL Antikörper (Internal Region)

Produktnummer ABIN374590

Produktdetails anti-DAZL Antikörper

Target: DAZL (Deleted in Azoospermia-Like (DAZL))

Bindungsspezifität: Internal Region

Reaktivität: Human, Maus, Ratte, Rind (Kuh), Hund

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser DAZL Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Sequenz: C-GNGPQKKSVD R

Spezifität: This antibody recognizes DAZL.

Kreuzreaktivität (Details): Species reactivity (expected):Rat, Canine, Bovine.
Species reactivity (tested):Human, Mouse.

Aufreinigung: Ammonium sulphate precipitation followed by antigen Affinity Chromatography using the immunizing peptide.

Immunogen: Synthetic peptide corresponding to the internal sequence of Human DAZL according to NP_001342.2 Genename: DAZL

Anwendungsinformationen

Applikationshinweise: Peptide ELISA: 1/64000. Western blot: Approx 35 kDa band observed in human ovary and human testes lysates(calculated MW of 33.5 kDa according to NP_001342.2). Recommended concentration: 0.1 - 0.3 μg/mL. Immunohistochemistry on paraffin sections: Mouse Testis shows cytoplasm staining ofspermatogonia. Additional staining is observed in the cytoplasm of elongatng spermatidsbut this additional staining is not in agreement with results obtained by another antibody(Ruggiuet al, Nature.1997 Sep 4,389(6646): 73-7. PMID: 9288969). Data kindly provided by J. S. Martins, MRC, Edinburgh, UK. Recommended concentration: 1 - 3 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C.

Details zu DAZL

Target: DAZL (Deleted in Azoospermia-Like (DAZL))

Andere Bezeichnung: DAZL (DAZL Produkte)

Hintergrund: DAZL is a member of the DAZ (deleted in azoospermia) family of RNA binding proteins. DAZL is expressed in fetal and adult testes and ovaries, and is believed to play a role in germ cell development. In adult germ cells, the expression of DAZL is localised to the cytoplasm. Mutations in this gene have been linked to severe spermatogenic failure and infertility in males.Synonyms: DAZ homolog, DAZ-like autosomal, DAZH, DAZL, DAZL1, DAZLA, Deleted in azoospermia-like, Deleted in azoospermia-like 1, SPGY-like-autosomal, SPGYLA

Gen-ID: 1618

NCBI Accession: NP_001342

UniProt: Q92904