PPY Antikörper (Internal Region)

Produktnummer ABIN374577

Der Ziege Polyklonal anti-PPY Antikörper (ABIN374577) detektiert spezifisch PPY in WB und IHC (p). Dieser Antikörper reagiert spezifisch mit Proben aus Human, Ratte, Maus und Hund.

Kurzübersicht für PPY Antikörper (Internal Region) (ABIN374577)

Target: PPY (Pancreatic Polypeptide (PPY))

Reaktivität: Human, Ratte, Maus, Hund

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser PPY Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-PPY Antikörper

Bindungsspezifität: Internal Region

Sequenz: C-TRPRYGKRHK EDT

Spezifität: This antibody recognizes Pancreatic prohormone.

Kreuzreaktivität (Details): Species reactivity (expected):Rat and Canine (Dog).
Species reactivity (tested):Human, Mouse.

Aufreinigung: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide

Immunogen: Synthetic peptide from the internal region of the protein sequence according to NP_002713.1. Genename: PPY

Anwendungsinformationen

Applikationshinweise: Peptide ELISA: Antibody Detection Limit Dilution: 1/32000. Western Blot: Preliminary experiments gave an approx 35-38 kDa band in human pancreaslysates after 1 μg/mL antibody staining. Please note that currently we cannot find anexplanation in the literature for the band we observe given the calculated size of 10.8 kDaaccording to NP_002713.1. The 38 kDa band was successfully blocked by incubation withthe immunizing peptide. Immunohistochemistry: 3 μg/mL. In paraffin embedded human pancreas, shows strong cytoplasmic staining of rare cellslocated at the periphery of islets of Langerhans.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu PPY

Target: PPY (Pancreatic Polypeptide (PPY))

Andere Bezeichnung: Pancreatic Prohormone

Hintergrund: Pancreatic hormone (PPY) is synthesized in pancreatic islets of Langerhans and acts as a regulator of pancreatic and gastrointestinal functions and may be important in the regulation of food intake. Plasma levels of this hormone have been shown to be reduced in conditions associated with increased food intake and elevated in anorexia nervosa. In addition, infusion of this hormone in obese rodents has shown to decrease weight gain.Synonyms: Obinepitide, PNP, PPY, Pancreatic polypeptide

Gen-ID: 5539

NCBI Accession: NP_002713

UniProt: P01298

Pathways: Feeding Behaviour