Dieser Ziege Polyklonal Antikörper detektiert spezifisch ADH1A in WB. Es zeigt Reaktivität gegenüber Proben von Human, Maus, Ratte, Pferd und Schimpanse.
This antibody is expected to recognize the alpha (ADH1A, NP_ 000658.1), the beta (ADH1B, NP_000659.2) and gamma (ADH1C, NP_000660.1) polypeptide variants of Human alcohol dehydrogenase.
Kreuzreaktivität (Details)
Species reactivity (expected):Mouse, Rat. Species reactivity (tested):Human.
Aufreinigung
Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide.
Immunogen
Peptide with sequence STAGKVMKCKA, from the N Terminus of the protein sequence according to NP_000658.1, NP_000659.2, NP_000660.1.
ADH1A
Reaktivität: Human
Wirt: Kaninchen
Polyclonal
FITC
Applikationshinweise
Peptide ELISA: 1/16000. Western Blot: 0.3-1.5 μg/mL. Approx 38 kDa band observed in Human Liver lysates(calculated MW of 39.8 kDa according to NP_000658.1, NP_000659.2 and 000660.1). Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Konzentration
0.5 mg/mL
Buffer
Tris saline, pH 7.3 containing 0.02 % Sodium Azide as presrevative and 0.5 % BSA as stabilizer.
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung
Avoid repeated freezing and thawing.
Lagerung
4 °C/-20 °C
Informationen zur Lagerung
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
ADH (Alcohol dehydrogenase) consists of a dimer of identical or nonidentical chains of three types, alpha, beta and gamma. Members of this enzyme family metabolize a wide variety of substrates, including ethanol, retinol, other aliphatic alcohols, hydroxysteroids, and lipid peroxidation products. There are 7 different ADH's isozymes in human: three belongs to class I: alpha, beta, and gamma, one to class II: pi, one to class III: chi, one to class IV: ADH7 and one to class V: ADH6.Synonyms: ADH1A, Alcohol dehydrogenase subunit alpha