FHL1 Antikörper (Internal Region)
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- Target Alle FHL1 Antikörper anzeigen
- FHL1 (Four and A Half LIM Domains 1 (FHL1))
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Bindungsspezifität
- Internal Region
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Reaktivität
- Human, Maus, Ratte, Hund, Rind (Kuh), Schwein
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Wirt
- Ziege
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Klonalität
- Polyklonal
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Konjugat
- Dieser FHL1 Antikörper ist unkonjugiert
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Applikation
- Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)
- Spezifität
- This antibody is expected to recognise isoforms 2 (NP_001440.1) and 5 (NP_001153171.1). Since June 2009 the second N residue in the immunizing peptide is no longer representative as it has been substituted by Q at position 284 of NP_001440.2. Variants NP_001440.2, NP_001153176.1 and NP_001153172.1 represent identical protein.
- Kreuzreaktivität (Details)
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Species reactivity (expected):Rat, Canine, Porcine and Bovine.
Species reactivity (tested):Human, Mouse. - Aufreinigung
- Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide.
- Immunogen
- Peptide with sequence CNKRFVFHNEQVY, from the internal region of the protein sequence according to NP_001440.1, NP_001153171.1. Genename: FHL1
- Top Product
- Discover our top product FHL1 Primärantikörper
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- Applikationshinweise
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Peptide ELISA: Limit Dilution: 1/32000. Western blot: 0.05-0.3 μg/mL. Detects a band of Approx 30 kDa in Human Muscle lysates(calculated MW of 31.0 kDa according to NP_001440). Immunohistochemistry: 2-2 μg/mL. In paraffin embedded Human Testis shows fibrousstaining in the cytoplasm of developing sperm cells.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Konzentration
- 0.5 mg/mL
- Buffer
- Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.
- Konservierungsmittel
- Sodium azide
- Vorsichtsmaßnahmen
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handhabung
- Avoid repeated freezing and thawing.
- Lagerung
- 4 °C/-20 °C
- Informationen zur Lagerung
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- Target
- FHL1 (Four and A Half LIM Domains 1 (FHL1))
- Andere Bezeichnung
- FHL1 (FHL1 Produkte)
- Synonyme
- fhl1 antikoerper, MGC89165 antikoerper, FHL1 antikoerper, DKFZp468O1927 antikoerper, fhl antikoerper, fhla antikoerper, zgc:92025 antikoerper, FHL-1 antikoerper, FHL1A antikoerper, FHL1B antikoerper, FLH1A antikoerper, KYOT antikoerper, SLIM antikoerper, SLIM-1 antikoerper, SLIM1 antikoerper, SLIMMER antikoerper, XMPMA antikoerper, KyoT antikoerper, Fhl1 antikoerper, four and a half LIM domains 1 antikoerper, four and a half LIM domains 1a antikoerper, Four and a half LIM domains protein 1 antikoerper, four and a half LIM domains 1 S homeolog antikoerper, fhl1 antikoerper, FHL1 antikoerper, fhl1a antikoerper, fhl1.S antikoerper, Fhl1 antikoerper
- Hintergrund
- FHL1 (SLIM1) is a LIM domain protein transcript that is preferentially expressed in postnatal skeletal muscle but little is known about its function in this tissue. LIM proteins, (named for 'LIN11, ISL1 and MEC3') are defined by the possession of a highly conserved double zinc finger motif called the LIM domain. FHL1 may have an involvement in muscle development or hypertrophy. Isoform 2 binds to RBP J and plays a negative regulatory role in the RBP J mediated transcription in mammalian systems.Synonyms: FHL-1, FHL1, Four and a half LIM domains protein 1, SLIM, SLIM-1, SLIM1, Skeletal Muscle LIM-protein 1
- Gen-ID
- 2273
- NCBI Accession
- NP_001153171
- UniProt
- Q13642
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