RAN Antikörper (N-Term)

Produktnummer ABIN374447

Der Ziege Polyklonal anti-RAN Antikörper wird verwendet zum Nachweis von RAN in Proben von Human, Maus, Ratte, Hund und Rind (Kuh). Er wurde validiert für WB und EIA.

Kurzübersicht für RAN Antikörper (N-Term) (ABIN374447)

Target: RAN (RAN, Member RAS Oncogene Family (RAN))

Reaktivität: Human, Maus, Ratte, Hund, Rind (Kuh)

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser RAN Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Enzyme Immunoassay (EIA)

Produktdetails anti-RAN Antikörper

Bindungsspezifität: N-Term

Sequenz: C-AAQGEPQVQF KLV

Spezifität: This antibody recognizes Ran (197-210).

Kreuzreaktivität (Details): Species reactivity (expected):Rat, Canine (Dog), Bovine.
Species reactivity (tested):Human, Mouse.

Aufreinigung: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide.

Immunogen: Peptide from the N-Terminus of the protein sequence according to NP_006316. Genename: RAN

Anwendungsinformationen

Applikationshinweise: Peptide ELISA: 1/16,000. Western Blot: 1-3 μg/mL. Detects a band of Approx 25 kDa (Predicted Molecular Weight: 25 kDa). Positive Control: A431 and Jurkat whole cell lysate.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu RAN

Target: RAN (RAN, Member RAS Oncogene Family (RAN))

Andere Bezeichnung: RAN

Hintergrund: Ran (ras-related nuclear protein) is a small GTP binding protein belonging to the RAS superfamily that is essential for the translocation of RNA and proteins through the nuclear pore complex. The Ran protein is also involved in control of DNA synthesis and cell cycle progression. Nuclear localization of Ran requires the presence of regulator of chromosome condensation 1 (RCC1). Mutations in Ran disrupt DNA synthesis. Because of its many functions, it is likely that Ran interacts with several other proteins. Ran regulates formation and organization of the microtubule network independently of its role in the nucleus-cytosol exchange of macromolecules. Ran could be a key signaling molecule regulating microtubule polymerization during mitosis. RCC1 generates a high local concentration of Ran-GTP around chromatin which, in turn, induces the local nucleation of microtubules. Ran is an androgen receptor (AR) coactivator that binds differentially with different lengths of polyglutamine within the androgen receptor. Polyglutamine repeat expansion in the AR is linked to Kennedy's disease (X-linked spinal and bulbar muscular atrophy). Ran coactivation of the AR diminishes with polyglutamine expansion within the AR, and this weak coactivation may lead to partial androgen insensitivity during the development of Kennedy's disease.Synonyms: ARA24, Androgen receptor-associated protein 24, GTP-binding nuclear protein Ran, GTPase Ran, Ras-like protein TC4, Ras-related nuclear protein

Molekulargewicht: 24.4 kDa (NP_006316.1)

Gen-ID: 5901, 9606

UniProt: P62826

Pathways: Regulatorische RNA Pathways, Intracellular Steroid Hormone Receptor Signaling Pathway, Protein targeting to Nucleus