CPEB1 Antikörper (C-Term)

Produktnummer ABIN374439

Der Ziege Polyklonal anti-CPEB1 Antikörper (ABIN374439) detektiert spezifisch CPEB1 in WB. Dieser Antikörper reagiert spezifisch mit Proben aus Human und Makake.

Kurzübersicht für CPEB1 Antikörper (C-Term) (ABIN374439)

Target: CPEB1 (Cytoplasmic Polyadenylation Element Binding Protein 1 (CPEB1))

Reaktivität: Human, Makake

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser CPEB1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Produktdetails anti-CPEB1 Antikörper

Bindungsspezifität: C-Term

Sequenz: C-PLMRNQKNRD SS

Spezifität: This antibody recognizes CPEB1.

Kreuzreaktivität (Details): Species reactivity (expected):Macaque.
Species reactivity (tested):Human.

Aufreinigung: Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide.

Immunogen: Peptide from the C Terminus of the protein sequence according to NP_085097.

Anwendungsinformationen

Applikationshinweise: Peptide ELISA: 1/128000. Western Blot: 0.5-2.0 μg/mL. Approx 65-70 kDa band observed in Human Brain lysates(predicted MW of 60 kDa according to NP_085097). Please note this band was always accompanied by a slightly stonger band at approx 40 kDaand further both bands could be blocked by incubation with the immunising peptide.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu CPEB1

Target: CPEB1 (Cytoplasmic Polyadenylation Element Binding Protein 1 (CPEB1))

Andere Bezeichnung: CPEB1

Hintergrund: The regulated translation of messenger RNA is essential for cell cycle progression, establishment of the body plan during early development, and modulation of key activities in the central nervous system. Cytoplasmic polyadenylation, which is one mechanism of controlling translation, is driven by CPEB1 (Cytoplasmic Polyadenylation Element Binding protein), a highly conserved, sequence specific RNA binding protein that binds to the cytoplasmic polyadenylation element (CPE) and modulates translational repression and mRNA localisation. CPEB1 can assemble bothe translational activation and repression complexes via its binding to the CPE. In both vertebrates and invertebrates, the expression of several maternal mRNAs is regulated by cytoplasmic polyadenylation. In Xenopus oocytes, where most of the biochemical details of this process have been examined, polyadenylation is controlled by CPEB1.Synonyms: CPE-BP1, CPE-binding protein 1, Cytoplasmic polyadenylation element-binding protein 1, h-CEBP, hCPEB-1

Gen-ID: 64506

NCBI Accession: NP_001073001

UniProt: Q9BZB8

Pathways: Synaptic Membrane