ACTR1B Antikörper (C-Term)

Produktnummer ABIN374429

Der Ziege Polyklonal anti-ACTR1B Antikörper wird verwendet zum Nachweis von ACTR1B in Proben von Human, Maus, Ratte, Hund und Rind (Kuh). Er wurde validiert für WB und IHC (p).

Kurzübersicht für ACTR1B Antikörper (C-Term) (ABIN374429)

Target: ACTR1B (ARP1 Actin-Related Protein 1 Homolog B, Centractin beta (ACTR1B))

Reaktivität: Human, Maus, Ratte, Hund, Rind (Kuh)

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser ACTR1B Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-ACTR1B Antikörper

Bindungsspezifität: C-Term

Sequenz: C-YEEDGSRAIH RKT

Spezifität: This antibody recognizes ARP1 homolog B.

Kreuzreaktivität (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Aufreinigung: Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide.

Immunogen: Peptide from the C Terminus of the protein sequence according to NP_005726. Genename: ACTR1B

Anwendungsinformationen

Applikationshinweise: Peptide ELISA: 1/128000. Western Blot: 0.5-2 μg/mL. Approx 45 kDa band observed in human heart and brain lysates(predicted MW of 45 kDa according to NP_005726). Immunohistochemistry: 3-5 μg/mL. In paraffin embedded Human Uterus shows fibrillar staining of cytoplasm in endometrialcells.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu ACTR1B

Target: ACTR1B (ARP1 Actin-Related Protein 1 Homolog B, Centractin beta (ACTR1B))

Andere Bezeichnung: Centractin beta

Hintergrund: In addition to conventional actins, there are several actin-related proteins. The ACTR1B protein and the ACTR1A protein are of equal length, and they share 90 % amino acid identity and 96 % amino acid similarity. ACTR1B is expressed in the cytosol as part of the dynactin complex. The dynactin complex has been shown to localize to multiple structures within the cell, including membrane organelles, the centrosome, spindle poles, and spindle pole microtubules during mitosis and prometaphase kinetochores. It regulates dynein mediated vesicle movement on microtubules as well as spindle assembly and cell division. ACTR1B appears to be involved in dynein-driven vesicle movement.Synonyms: ACTR1B, ARP1B, Actin-related protein 1B, CTRN2

Gen-ID: 10120

NCBI Accession: NP_005726

UniProt: P42025