BAF53A Antikörper (C-Term)

Produktnummer ABIN374427

Produktdetails anti-BAF53A Antikörper

Target: BAF53A (Actin-Like 6A (BAF53A))

Bindungsspezifität: C-Term

Reaktivität: Human, Maus, Ratte, Rind (Kuh), Hund

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser BAF53A Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Sequenz: YEEGGKQCVE RKCP

Spezifität: This antibody detects ACTL6A / BAF53 at C-term.

Kreuzreaktivität (Details): Species reactivity (expected):Mouse, Rat, Dog, CowSpecies reactivity (tested):Human

Aufreinigung: Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

Immunogen: Peptide from C Terminus of the protein sequence according to NP_004292.1, NP_817126.1, NP_057272.1. Genename: ACTL6A / ACTL6B

Anwendungsinformationen

Applikationshinweise: Peptide ELISA: Limit dilution 1: 64000. Western Blot: 1-3 μg/mL. Approx 48 kDa band observed in Hela lysates (predicted MW of48 kDa according to NP_057272, NP_004292).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C.

Details zu BAF53A

Target: BAF53A (Actin-Like 6A (BAF53A))

Andere Bezeichnung: ACTL6A / BAF53 (BAF53A Produkte)

Synonyme: ACTL6 antikoerper, ARPN-BETA antikoerper, Arp4 antikoerper, BAF53A antikoerper, INO80K antikoerper, 2810432C06Rik antikoerper, AI851094 antikoerper, ARP4 antikoerper, Actl6 antikoerper, Baf53a antikoerper, C79802 antikoerper, baf53a antikoerper, fb52e06 antikoerper, fl26e12 antikoerper, chunp6900 antikoerper, wu:fb52e06 antikoerper, wu:fl26e12 antikoerper, actl6 antikoerper, MGC75671 antikoerper, RGD1307747 antikoerper, ACTL6A antikoerper, ATARP4 antikoerper, F15H18.8 antikoerper, F15H18_8 antikoerper, actin-related protein 4 antikoerper, Actin-like protein 4 antikoerper, CaO19.5623 antikoerper, actin antikoerper, actl6a antikoerper, actl6a.L antikoerper, arp4 antikoerper, arpn-beta antikoerper, ino80k antikoerper, actin like 6A antikoerper, actin-like 6A antikoerper, actin-related protein 4 antikoerper, Arp4p antikoerper, actin like 6A S homeolog antikoerper, ACTL6A antikoerper, Actl6a antikoerper, actl6a antikoerper, ARP4 antikoerper, actl6a.S antikoerper

Hintergrund: ACTL6A is a family member of actin-related proteins (ARPs), which share significant amino acid sequence identity to conventional actins. Both actins and ARPs have an actin fold, which is an ATP-binding cleft, as a common feature. The ARPs are involved in diverse cellular processes, including vesicular transport, spindle orientation, nuclear migration and chromatin remodeling. ACTL6A is a 53 kDa subunit of the BAF (BRG1/brm-associated factor) complex in mammals, which is functionally related to SWI/SNF complex in S. cerevisiae and Drosophila, the latter is thought to facilitate transcriptional activation of specific genes by antagonizing chromatin-mediated transcriptional repression. Together with beta-actin, it is required for maximal ATPase activity of BRG1, and for the association of the BAF complex with chromatin/matrix. It is required for maximal ATPase activity of SMARCA4/BRG1 and for association of the SMARCA4/BRG1 containing remodelling complex BAF with chromatin/nuclear matrix. ACTL6A is a component of the NuA4 histone acetyltransferase (HAT) complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histone H4 and H2A. This modification may both alter nucleosome - DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair. NuA4 may also play a direct role in DNA repair when recruited to sites of DNA damage.Synonyms: 53 kDa BRG1-associated factor A, Actin-like protein 6A, Actin-related protein Baf53a, ArpNbeta, BAF53A, INO80 complex subunit K, INO80K

Gen-ID: 86

NCBI Accession: NP_004292

UniProt: O96019

Pathways: Chromatin Binding, Photoperiodism