PITPNM1 Antikörper (C-Term)

Produktnummer ABIN374422

Dieser Ziege Polyklonal Antikörper detektiert spezifisch PITPNM1 in WB und IF. Es zeigt Reaktivität gegenüber Proben von Human, Maus, Rind (Kuh) und Hund.

Kurzübersicht für PITPNM1 Antikörper (C-Term) (ABIN374422)

Target: PITPNM1 (Phosphatidylinositol Transfer Protein, Membrane-Associated 1 (PITPNM1))

Reaktivität: Human, Maus, Rind (Kuh), Hund

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser PITPNM1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF)

Produktdetails anti-PITPNM1 Antikörper

Bindungsspezifität: C-Term

Sequenz: C-KARSISLKLD SEE

Spezifität: This antibody detects NIR2 at C-term and is expected to recognise both reported isoforms.

Kreuzreaktivität (Details): Species reactivity (expected):Human, Dog, Cow.
Species reactivity (tested):Mouse.

Aufreinigung: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide

Immunogen: Peptide from the C-Terminus of the protein sequence according to NP_004901.2, NP_001124320.1. Genename: PITPNM1

Anwendungsinformationen

Applikationshinweise: Peptide ELISA: Limit dilution 1: 8000. Western blot: Customer's experiments gave a band at approx 160 kDa in lysates of a MouseMacrophage cell line after 2 μg/mL antibody staining (calculated MW of 130 kDa accordingto NP_004901.1). In ICC the customer found staining of vesicular cytoplasm in the samecell line.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu PITPNM1

Target: PITPNM1 (Phosphatidylinositol Transfer Protein, Membrane-Associated 1 (PITPNM1))

Andere Bezeichnung: NIR2

Hintergrund: NIR2 belongs to a family of proteins that share homology with the Drosophila retinal degeneration B (rdgB) protein. Nir2, a human homolog of Drosophila melanogaster retinal degeneration B protein, is essential for cytokinesis. A specific Thr residue in the Nir2 PI-transfer domain provides a regulatory site for targeting to lipid droplets. This may affect intracellular lipid trafficking and distribution & explain the dominant effect of the RdgB-T59E mutant on retinal degeneration.Synonyms: DRES9, Membrane-associated phosphatidylinositol transfer protein 1, NIR2, PITPNM, PITPNM1

Gen-ID: 9600

NCBI Accession: NP_004901

UniProt: O00562