VPS35 Antikörper (C-Term)

Produktnummer ABIN374403

Der Ziege Polyklonal anti-VPS35 Antikörper (ABIN374403) detektiert spezifisch VPS35 in WB, IF, IHC (p) und EIA. Dieser Antikörper reagiert spezifisch mit Proben aus Human, Maus, Ratte und Rind (Kuh).

Kurzübersicht für VPS35 Antikörper (C-Term) (ABIN374403)

Target: VPS35 (Vacuolar Protein Sorting 35 (VPS35))

Reaktivität: Human, Maus, Ratte, Rind (Kuh)

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser VPS35 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Produktdetails anti-VPS35 Antikörper

Bindungsspezifität: C-Term

Sequenz: C-SPESEGPIYE GLIL

Spezifität: This antibody detects VPS35 / MEM3 at C-term.

Kreuzreaktivität (Details): Species reactivity (expected):Mouse, Rat, Cow.
Species reactivity (tested):Human.

Aufreinigung: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide

Immunogen: Syntehtic peptide from the C-Terminus of Human VPS35 (NP_060676.2). Genename: VPS35

Anwendungsinformationen

Applikationshinweise: Peptide ELISA: 1/16000. Western blot: Approx 90 kDa band observed in Human Brain and Testis lysates (predictedMW of 90 kDa according to NP_060676.2). Recommended concentration: 1 - 3 μg/mL. Immunohistochemistry on paraffin sections: Human Testis, shows positive staining inLeydig cells, and cytoplasmic staining (dot-like) of some epithelial cells of the epididymisand of the rete testis. Immunoflurescence: Staining of VPS35 (red) in human melanoma cells 1205LU: Please seeScott KL et al. , Nature.2009 Jun 25,459(7250): 1085-90. , PMID: 19553991.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu VPS35

Target: VPS35 (Vacuolar Protein Sorting 35 (VPS35))

Andere Bezeichnung: VPS35 / MEM3

Hintergrund: Synonyms: Maternal-embryonic 3, TCCCTA00141, Vacuolar protein sorting-associated protein 35, Vesicle protein sorting 35

Gen-ID: 55737

NCBI Accession: NP_060676

UniProt: Q96QK1