SIPA1 Antikörper (C-Term)

Produktnummer ABIN374401

Dieser Ziege Polyklonal Antikörper detektiert spezifisch SIPA1 in WB. Es zeigt Reaktivität gegenüber Proben von Human.

Kurzübersicht für SIPA1 Antikörper (C-Term) (ABIN374401)

Target: SIPA1 (Signal-Induced Proliferation-Associated 1 (SIPA1))

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser SIPA1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Produktdetails anti-SIPA1 Antikörper

Bindungsspezifität: C-Term

Sequenz: C-ASKQLGSPTA DLA

Spezifität: This antibody detects SPA1 / SIPA1 at C-term.

Kreuzreaktivität (Details): Species reactivity (tested):Human

Aufreinigung: Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

Immunogen: Peptide from C Terminus of the protein sequence according to NP_694985.28, NP_006738.3 Genename: SIPA1

Anwendungsinformationen

Applikationshinweise: Peptide ELISA: Limit dilution 1: 32000. Western blot: 1-3 μg/mL. Approx.150 kDa band observed in Human lysates ofEBV-immortilized lymphoblastoid (calculated MW of 110 kDa according to NP_694985.28and NP_006738.2). The observed molecular weight corresponds to earlier findings inliterature with different antibodies (Kurachi et al, J Biol Chem.1997 Oct31,272(44): 28081-8, PMID: 9346962). An additional band of 55-60 kDa was consistentlyobserved, however this band was not blocked by the immunizing peptide and it istherefore a non-specific signal.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C.

Details zu SIPA1

Target: SIPA1 (Signal-Induced Proliferation-Associated 1 (SIPA1))

Andere Bezeichnung: SIPA1 / SPA1

Hintergrund: Synonyms: GTPase-activating protein Spa-1, Signal-induced proliferation-associated protein 1, Sipa-1, Spa-1, p130 SPA-1

Gen-ID: 6494

UniProt: Q96FS4

Pathways: Response to Water Deprivation