GRAF Antikörper (C-Term)

Produktnummer ABIN374371

Dieses Ziege Polyklonal-Antikörper erkennt spezifisch GRAF in WB. Er zeigt eine Reaktivität gegenüber Human, Maus, Ratte, Rind (Kuh) und Hund.

Kurzübersicht für GRAF Antikörper (C-Term) (ABIN374371)

Target: GRAF (ARHGAP26) (rho GTPase Activating Protein 26 (ARHGAP26))

Reaktivität: Human, Maus, Ratte, Rind (Kuh), Hund

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser GRAF Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Produktdetails anti-GRAF Antikörper

Bindungsspezifität: C-Term

Sequenz: C-KTGLIPENYV EFL

Spezifität: This antibody detects ARHGAP26/OPHN1L at C-term. It is expected to recognise both reported isoforms.

Kreuzreaktivität (Details): Species reactivity (expected):Mouse, Rat, Canine (Dog) and Bovine (Cow).
Species reactivity (tested):Human.

Aufreinigung: Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide

Immunogen: Peptide from C-Terminus of the protein sequence according to NP_055886.1, NP_001129080.1. Genename: ARHGAP26

Anwendungsinformationen

Applikationshinweise: Peptide ELISA: Limit dilution 1/64000. Western blot: 0.3-1 μg/mL. Approx 90 kDa band observed in Human Heart lysates(calculated MW of 92.0 kDa according to NP_055886.1). An additional strong band ofunknown identity was also consistently observed at 10 kDa. This band was successfullyblocked by incubation with the immunising peptide. We would appreciate any feedbackfrom people in the field - have any results been reported with other antibodies/lysates?Have any further splice variants/modified forms been reported?
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: Tris saline, pH 7.3 with 0.02 % Sodium Azide and 0.5 % BSA

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C.

Details zu GRAF

Target: GRAF (ARHGAP26) (rho GTPase Activating Protein 26 (ARHGAP26))

Andere Bezeichnung: ARHGAP26 / OPHN1L

Hintergrund: Synonyms: GRAF, GTPase regulator associated with focal adhesion kinase, KIAA0621, Oligophrenin-1-like protein, Rho GTPase-activating protein 26, Rho-type GTPase-activating protein 26

Gen-ID: 23092

NCBI Accession: NP_055886

UniProt: Q9UNA1