Anillin Antikörper (C-Term)

Produktnummer ABIN374360

Der Ziege Polyklonal anti-Anillin Antikörper wird verwendet zum Nachweis von Anillin in Proben von Human, Maus und Rind (Kuh). Er wurde validiert für WB, IHC (p) und EIA.

Kurzübersicht für Anillin Antikörper (C-Term) (ABIN374360)

Target: Anillin (ANLN) (Anillin, Actin Binding Protein (ANLN))

Reaktivität: Human, Maus, Rind (Kuh)

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser Anillin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Produktdetails anti-Anillin Antikörper

Bindungsspezifität: C-Term

Sequenz: WQPDACYKPI GKP

Spezifität: This antibody detects Anillin / ANLN at C-term.

Kreuzreaktivität (Details): Species reactivity (expected):Mouse, Cow.
Species reactivity (tested):Human.

Aufreinigung: Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

Immunogen: Peptide from C Terminus of the protein sequence according to NP_061155.2. Genename: ANLN

Anwendungsinformationen

Applikationshinweise: Peptide ELISA: Limit dilution 1: 64000. Western blot: Experiments gave an approx 75 kDa band in Mouse Brain lysates after 0.3 μg/mL antibody staining. It was successfully blocked by incubation with the immunizingpeptide. Immunohistochemistry: 3-10 μg/mL. In paraffin embedded Human Kidney shows staining ofnuclei in some cells of renal tubules.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C.

Details zu Anillin

Target: Anillin (ANLN) (Anillin, Actin Binding Protein (ANLN))

Andere Bezeichnung: Anillin / ANLN

Hintergrund: Anillin is required for cytokinesis. It is essential for the structural integrity of the cleavage furrow and for completion of cleavage furrow ingression.Synonyms: Actin-binding protein anillin

Gen-ID: 54443

NCBI Accession: NP_061155

UniProt: Q9NQW6