FBXO43 Antikörper (C-Term)

Produktnummer ABIN374351

Der Ziege Polyklonal Anti-FBXO43-Antikörper wurde für WB, IF, EIA und FACS validiert. Er ist geeignet, FBXO43 in Proben von Human, Maus, Ratte, Rind (Kuh), Hund und Schwein zu detektieren.

Kurzübersicht für FBXO43 Antikörper (C-Term) (ABIN374351)

Target: FBXO43 (F-Box Protein 43 (FBXO43))

Reaktivität: Human, Maus, Ratte, Rind (Kuh), Hund, Schwein

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser FBXO43 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Enzyme Immunoassay (EIA), Flow Cytometry (FACS)

Produktdetails anti-FBXO43 Antikörper

Bindungsspezifität: C-Term

Sequenz: C-PGSAQSKRNL KRL

Spezifität: Recognizes EMI2.

Kreuzreaktivität (Details): Species reactivity (expected):Human, Mouse, Canine, Pig, Cow.
Species reactivity (tested):Rat.

Aufreinigung: Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide

Immunogen: Peptide C Terminus of the protein sequence according to NP_001025031.2. Genename: FBXO43

Anwendungsinformationen

Applikationshinweise: Peptide ELISA: Limit dilution 1: 16000. Western blot: 1-3 μg/mL. Has been successfully used on Rat Pachytene Spermatocytesshowing a 71 kDa band (Perrard et al, PLoS One.2009 Oct 5,4(10): e7237. PMID: 19802389). This product has been successfully used in Immunofluorescence and Flow Cytometry onRat meiotic Testis cells (5-10 μg/mL): (Perrard et al, PLoS One.2009 Oct 5,4(10): e7237. PMID: 19802389).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: Tris saline, 0.02 % Sodium Azide, pH 7.3 with 0.5 % BSA

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu FBXO43

Target: FBXO43 (F-Box Protein 43 (FBXO43))

Andere Bezeichnung: FBXO43

Hintergrund: Members of the F-box protein family, such as Fbp5B, are characterized by an approximately 40-amino acid F-box motif. SCF complexes, formed by SKP1 (MIM 601434), cullin (see CUL1, MIM 603134), and F-box proteins, act as protein-ubiquitin ligases. F-box proteins interact with SKP1 through the F box, and they interact with ubiquitination targets through other protein interaction domains.Synonyms: EMI2, Endogenous meiotic inhibitor 2, F-box only protein 43, FBP5B

Gen-ID: 286151

NCBI Accession: NP_001025031

UniProt: Q4G163