FBXO43 Antikörper (C-Term)

Produktnummer ABIN374351

Der Ziege-Polyklonal Anti-FBXO43-Antikörper wurde für WB, IF, EIA und FACS validiert. Er ist geeignet zum Nachweis von FBXO43 in Proben aus Human, Maus, Ratte, Rind (Kuh), Hund und Schwein. }

Kurzübersicht für FBXO43 Antikörper (C-Term) (ABIN374351)

Target: FBXO43 (F-Box Protein 43 (FBXO43))

Reaktivität: Human, Maus, Ratte, Rind (Kuh), Hund, Schwein

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser FBXO43 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Enzyme Immunoassay (EIA), Flow Cytometry (FACS)

Produktdetails anti-FBXO43 Antikörper

Bindungsspezifität: C-Term

Sequenz: C-PGSAQSKRNL KRL

Spezifität: Recognizes EMI2.

Kreuzreaktivität (Details): Species reactivity (expected):Human, Mouse, Canine, Pig, Cow.
Species reactivity (tested):Rat.

Aufreinigung: Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide

Immunogen: Peptide C Terminus of the protein sequence according to NP_001025031.2. Genename: FBXO43

Anwendungsinformationen

Applikationshinweise: Peptide ELISA: Limit dilution 1: 16000. Western blot: 1-3 μg/mL. Has been successfully used on Rat Pachytene Spermatocytesshowing a 71 kDa band (Perrard et al, PLoS One.2009 Oct 5,4(10): e7237. PMID: 19802389). This product has been successfully used in Immunofluorescence and Flow Cytometry onRat meiotic Testis cells (5-10 μg/mL): (Perrard et al, PLoS One.2009 Oct 5,4(10): e7237. PMID: 19802389).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: Tris saline, 0.02 % Sodium Azide, pH 7.3 with 0.5 % BSA

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu FBXO43

Target: FBXO43 (F-Box Protein 43 (FBXO43))

Andere Bezeichnung: FBXO43

Hintergrund: Members of the F-box protein family, such as Fbp5B, are characterized by an approximately 40-amino acid F-box motif. SCF complexes, formed by SKP1 (MIM 601434), cullin (see CUL1, MIM 603134), and F-box proteins, act as protein-ubiquitin ligases. F-box proteins interact with SKP1 through the F box, and they interact with ubiquitination targets through other protein interaction domains.Synonyms: EMI2, Endogenous meiotic inhibitor 2, F-box only protein 43, FBP5B

Gen-ID: 286151

NCBI Accession: NP_001025031

UniProt: Q4G163