PPP1R8 Antikörper (C-Term)

Produktnummer ABIN374344

Produktdetails anti-PPP1R8 Antikörper

Target: PPP1R8 (Protein Phosphatase 1, Regulatory Subunit 8 (PPP1R8))

Bindungsspezifität: C-Term

Reaktivität: Human, Maus, Ratte, Rind (Kuh), Hund

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser PPP1R8 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Sequenz: C-EAWPGKKPTP SLLI

Spezifität: This antibody detects NIPP-1 at C-term. It is expected to recognise all three Human isoforms of this protein.

Kreuzreaktivität (Details): Species reactivity (expected):Mouse, Rat, Dog, Cow.
Species reactivity (tested):Human.

Aufreinigung: Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide

Immunogen: Peptide from C Terminus of the protein sequence according to NP_002704.1, NP_054829.2, NP_612568.1. Genename: PPP1R8

Anwendungsinformationen

Applikationshinweise: Peptide ELISA: Limit dilution 1: 32000. Western blot: 1-2 μg/mL. Approx 40 kDa band observed in Human Lung and H460 cellextracts (predicted MW of 40 kDa according to NP_054829).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: Tris buffered saline, pH ~7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu PPP1R8

Target: PPP1R8 (Protein Phosphatase 1, Regulatory Subunit 8 (PPP1R8))

Andere Bezeichnung: NIPP-1 (PPP1R8 Produkte)

Hintergrund: Inhibitor subunit of the major nuclear protein phosphatase 1 (PP1). It has RNA binding activity but does not cleave RNA and may target PP1 to RNA associated substrates. May also be involved in pre mRNA splicing. Binds DNA and might act as a transcriptional repressor. Seems to be required for cell proliferation.Synonyms: ARD1, NIPP1, Nuclear inhibitor of protein phosphatase 1, PPP1R8

Gen-ID: 5511

NCBI Accession: NP_002704

UniProt: Q12972