FOXC1 Antikörper (C-Term)

Produktnummer ABIN374336

Es sind 3+ Publikationen für dieses Produkt verfügbar. Der Ziege Polyklonal anti-FOXC1 Antikörper wird verwendet zum Nachweis von FOXC1 in Proben von Human, Maus und Zebrafisch (Danio rerio). Er wurde validiert für WB, IHC (p), ChIP und EIA.

Kurzübersicht für FOXC1 Antikörper (C-Term) (ABIN374336)

Target: FOXC1 (Forkhead Box C1 (FOXC1))

Reaktivität: Human, Maus, Zebrafisch (Danio rerio)

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser FOXC1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Chromatin Immunoprecipitation (ChIP), Enzyme Immunoassay (EIA)

Produktdetails anti-FOXC1 Antikörper

Bindungsspezifität: C-Term

Sequenz: RTSGAFVYDC SKF

Spezifität: This antibody detects FOXC1 / FKHL7 / FREAC3 at C-term.

Kreuzreaktivität (Details): Species reactivity (tested):Human, Mouse, Zebrafish.

Aufreinigung: Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide

Immunogen: Peptide from C-Terminus of the protein sequence according to NP_001444.2. Genename: FOXC1

Anwendungsinformationen

Applikationshinweise: Peptide ELISA: Limit dilution 1: 32000. Western blot: 0.5-1.5 μg/mL. Approx 55 kDa band observed in Human Bone Marrow lysates(calculated MW of 56.5 kDa according to NP_001444.1). Immunohistochemistry: 3 μg/mL. In paraffin embedded Human Kidney shows nuclearstaining of some cells in the glomeruli.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C.

Referenzen für anti-FOXC1 Antikörper (ABIN374336)

Doucette, Footz, Walter: "FOXC1 Regulates Expression of Prostaglandin Receptors Leading to an Attenuated Response to Latanoprost." in: Investigative ophthalmology & visual science, Vol. 59, Issue 6, pp. 2548-2554, (2019) (PubMed).

Fatima, Wang, Uchida, Norden, Liu, Culver, Dietz, Culver, Millay, Mukouyama, Kume: "Foxc1 and Foxc2 deletion causes abnormal lymphangiogenesis and correlates with ERK hyperactivation." in: The Journal of clinical investigation, Vol. 126, Issue 7, pp. 2437-51, (2017) (PubMed).

Ito, Goping, Berry, Walter: "Dysfunction of the stress-responsive FOXC1 transcription factor contributes to the earlier-onset glaucoma observed in Axenfeld-Rieger syndrome patients." in: Cell death & disease, Vol. 5, pp. e1069, (2014) (PubMed).

Details zu FOXC1

Target: FOXC1 (Forkhead Box C1 (FOXC1))

Andere Bezeichnung: FOXC1 / FKHL7 / FREAC3

Hintergrund: FOXC1 belongs to the forkhead family of transcription factors which is characterized by a distinct DNA-binding forkhead domain. The specific function of this gene has not yet been determined, however, it has been shown to play a role in the regulation of embryonic and ocular development. Mutations in this gene cause various glaucoma phenotypes including primary congenital glaucoma, autosomal dominant iridogoniodysgenesis anomaly, and Axenfeld-Rieger anomaly.Synonyms: FREAC-3, Forkhead box protein C1, Forkhead-related protein FKHL7, Forkhead-related transcription factor 3

Molekulargewicht: 56.8kDa (NP_001444.1).

Gen-ID: 2296

NCBI Accession: NP_001444

UniProt: Q12948

Pathways: Chromatin Binding, Glycosaminoglycan Metabolic Process