STRADB Antikörper (C-Term)

Produktnummer ABIN374305

Produktdetails anti-STRADB Antikörper

Target: STRADB (STE20-Related Kinase Adaptor beta (STRADB))

Bindungsspezifität: C-Term

Reaktivität: Human, Rind (Kuh), Hund

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser STRADB Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Spezifität: This antibody detects STRAD beta / ILPIP at C-term. This is expected to recognise both human isoforms: ILPIP-alpha and ILPIP-beta.

Kreuzreaktivität (Details): Species reactivity (expected):Canine, Bovine.
Species reactivity (tested):Human

Aufreinigung: Affinity chromatography

Immunogen: Peptide with sequence CDFPDEKDSYWEF, from the C Terminus of the protein sequence Genename: STRADB

Anwendungsinformationen

Applikationshinweise: Peptide ELISA: 1/32000. Western blot: 1 - 3 μg/mL. Approx 35 kDa band observed in Human Heart, Liver and Musclelysates (calculated MW of 47.5 kDa according to NP_061041.2 (alpha isoform) and 31.5 kDaaccording to AAF71042.1(beta isoform). In transfected HEK293 transiently expressing ILPIPa band of approx.45 kDa is observed. This band is not observed in the non-transfectedHEK293. Immunohistochemistry on paraffin sections: 3 - 5 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu STRADB

Target: STRADB (STE20-Related Kinase Adaptor beta (STRADB))

Andere Bezeichnung: STRAD beta / ILPIP (STRADB Produkte)

Hintergrund: Synonyms: ALS2CR2, CALS-21, PRO1038, Pseudokinase ALS2CR2, STE20-related kinase adapter protein beta, STRADB

Gen-ID: 55437

NCBI Accession: NP_061041

UniProt: Q9C0K7

Pathways: AMPK Signaling