Midline 2 Antikörper (C-Term)

Produktnummer ABIN374299

Produktdetails anti-Midline 2 Antikörper

Target: Midline 2 (MID2)

Bindungsspezifität: C-Term

Reaktivität: Human, Maus, Ratte, Hund, Rind (Kuh), Schwein

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser Midline 2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Sequenz: QESPYVSGMK TCH

Spezifität: This antibody detects RNF60 / MID2 at C-term.

Kreuzreaktivität (Details): Species reactivity (expected):Rat, Dog, Pig, CowSpecies reactivity (tested):Human, Mouse

Aufreinigung: Amonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

Immunogen: Peptide from C Terminus of the protein sequence according to NP_036348.2, NP_438112.2 Genename: MID2

Anwendungsinformationen

Applikationshinweise: Peptide ELISA: Lmit dilution 1: 32000. Western bot: 1-3 μml. Approx 80 kDa band observed in mouse heart lysates (calculated MWof 81.0 kDa according to NP_036348.1 and 77.0 kDa according to NP_438112.1). Immunohistochemistry: 5-10 μg/mL. In paraffin embedded Human Kidney shows strongstaining in cytoplasm and brush border of some epithelial cells of proximal convolutedtubules.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C.

Details zu Midline 2

Target: Midline 2 (MID2)

Andere Bezeichnung: MID2 / RNF60 (MID2 Produkte)

Synonyme: MID2 antikoerper, fxy2 antikoerper, rnf60 antikoerper, trim1 antikoerper, FXY2 antikoerper, RNF60 antikoerper, TRIM1 antikoerper, Trim1 antikoerper, RGD1565674 antikoerper, midline 2 antikoerper, midline 2 L homeolog antikoerper, MID2 antikoerper, mid2 antikoerper, mid2.L antikoerper, Mid2 antikoerper

Hintergrund: Synonyms: FXY2, Midin-2, Midline defect 2, Midline-2, Probable E3 ubiquitin-protein ligase MID2, RING finger protein 60, TRIM1, Tripartite motif-containing protein 1

Gen-ID: 11043

NCBI Accession: NP_036348

UniProt: Q9UJV3