BAZ2B Antikörper (C-Term)

Produktnummer ABIN374295

Dieses Anti-BAZ2B-Antikörper ist ein Ziege Polyklonal-Antikörper zur Detektion von BAZ2B in WB und IHC (p). Geeignet für Human, Maus, Rind (Kuh) und Ratte.

Kurzübersicht für BAZ2B Antikörper (C-Term) (ABIN374295)

Target: BAZ2B (Bromodomain Adjacent To Zinc Finger Domain, 2B (BAZ2B))

Reaktivität: Human, Maus, Rind (Kuh), Ratte

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser BAZ2B Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-BAZ2B Antikörper

Bindungsspezifität: C-Term

Sequenz: C-YFEKKWTDTF KVS

Spezifität: This antibody detects BAZ2B at C-term.

Kreuzreaktivität (Details): Species reactivity (expected):Mouse, Rat, CowSpecies reactivity (tested):Human

Aufreinigung: Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

Immunogen: Peptide from C Terminus of the protein sequence according to NP_038478.2 Genename: BAZ2B

Anwendungsinformationen

Applikationshinweise: Peptide ELISA: Limit dilution 1: 4000. Western blot: Preliminary experiments gave an approx 35 kDa band in Testis and Brainlysates after 0.3 μg/mL antibody staining. Please note that currently we cannot find anexplanation in the literature for the band we observe given the calculated size of 225 kDaaccording to NP_038478. The 35 kDa band was successfully blocked by incubation with theimmunizing peptide. We would appreciate any feedback from people in the field - have anyresults been reported with other antibodies/lysates? Have any further splicevariants/modified forms been reported?Immunohistochemistry: 1-2 μg/mL. In paraffin embedded Human Small Intestine showsprimarily nuclear staining in a number of cells of the lamina propria.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C.

Details zu BAZ2B

Target: BAZ2B (Bromodomain Adjacent To Zinc Finger Domain, 2B (BAZ2B))

Andere Bezeichnung: BAZ2B

Hintergrund: BAZ2B may play a role in transcriptional regulation interacting with ISWI. It is expressed at varying levels in several tissues, whereas a smaller transcript was expressed specifically in testis.Synonyms: Bromodomain adjacent to zinc finger domain protein 2B, KIAA1476, hWALp4

Gen-ID: 29994

NCBI Accession: NP_038478

UniProt: Q9UIF8