IRF8 Antikörper (C-Term)

Produktnummer ABIN374256

Dieses Anti-IRF8-Antikörper ist ein Ziege Polyklonal-Antikörper zur Detektion von IRF8 in WB. Geeignet für Human, Maus, Ratte und Rind (Kuh).

Kurzübersicht für IRF8 Antikörper (C-Term) (ABIN374256)

Target: IRF8 (Interferon Regulatory Factor 8 (IRF8))

Reaktivität: Human, Maus, Ratte, Rind (Kuh)

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser IRF8 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Produktdetails anti-IRF8 Antikörper

Bindungsspezifität: C-Term

Sequenz: C-QRSFFRENQQ ITV

Spezifität: This antibody detects IRF8 / ICSBP1 at C-term.

Kreuzreaktivität (Details): Species reactivity (expected):Mouse, Rat, CowSpecies reactivity (tested):Human

Aufreinigung: Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

Immunogen: Peptide from C Terminus of the protein sequence according to NP_002154.1 Genename: IRF8

Anwendungsinformationen

Applikationshinweise: Peptide ELISA: Limit dilution 1: 8000. Western blot: 0.5-2 μg/mL. Approx 45-50 kDa band seen in K562 lysate.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C.

Details zu IRF8

Target: IRF8 (Interferon Regulatory Factor 8 (IRF8))

Andere Bezeichnung: IRF8 / ICSBP1

Hintergrund: Interferon regulatory factor 8 (IRF8) / Interferon consensus sequence binding protein (ICSBP) is a transcription factor of the interferon (IFN) regulatory factor (IRF) family. Proteins of this family are composed of a conserved DNA binding domain in the N terminal region and a divergent C terminal region that serves as the regulatory domain. The IRF family proteins bind to the IFN stimulated response element (ISRE) and regulate expression of genes stimulated by type I IFNs, namely IFN alpha and IFN beta. IRF family proteins also control expression of IFN alpha and IFN beta regulated genes that are induced by viral infection. IRF8 specifically binds to the upstream regulatory region of type I IFN and IFN inducible MHC class I genes (the interferon consensus sequence (ICS)). Unlike IRF3, IRF8 appears to act as a negative regulator of IFN induced genes in most cases, but IRF8 mediates activation of NF kappaB by the toll like receptor 9 (TLR9) after stimulation by unmethylated CpG DNA in dendritic cells. Finally, it has been shown that IRF8 decreases bcl 2 expression and thus may play a role in chronic myelogenous leukemia.Synonyms: IRF-8, Interferon consensus sequence-binding protein, Interferon regulatory factor 8

Gen-ID: 3394

NCBI Accession: NP_002154

UniProt: Q02556

Pathways: Cellular Response to Molecule of Bacterial Origin