PEBP1 Antikörper (C-Term)

Produktnummer ABIN374198

Der Ziege Polyklonal anti-PEBP1 Antikörper wird verwendet zum Nachweis von PEBP1 in Proben von Human, Maus, Ratte, Rind (Kuh) und Hund. Er wurde validiert für WB, IHC (p) und EIA.

Kurzübersicht für PEBP1 Antikörper (C-Term) (ABIN374198)

Target: PEBP1 (Phosphatidylethanolamine Binding Protein 1 (PEBP1))

Reaktivität: Human, Maus, Ratte, Rind (Kuh), Hund

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser PEBP1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Produktdetails anti-PEBP1 Antikörper

Bindungsspezifität: C-Term

Sequenz: C-DYVPKLYEQL SGK

Spezifität: This antibody detects PBP / RKIP at C-term.

Kreuzreaktivität (Details): Species reactivity (expected):Mouse, Rat, Canine (Dog) and Cow.
Species reactivity (tested):Human, Rat.

Aufreinigung: Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide.

Immunogen: Peptide from C Terminus of the protein sequence according to NP_002558.1 Genename: PEBP1

Anwendungsinformationen

Applikationshinweise: Peptide ELISA: Limit Dilution: 1/16000. Western blot: 0.01-0.03 μg/mL. Approx 22 kDa band observed in lysates of Human Kidneyfibroblast HEK293, Human lung carcinoma A549 and Human Hepatoblastoma HepG2 celllines (calculated MW of 21.2 kDa according to NP_002558.1). Immunohistochemistry: 1-2 μg/mL. In paraffin embedded Human Brain (Cortex) shows staining near sectect nuclei.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu PEBP1

Target: PEBP1 (Phosphatidylethanolamine Binding Protein 1 (PEBP1))

Andere Bezeichnung: PEBP1 / RKIP

Hintergrund: PBP, also known as RKIP (RAF kinase inhibitor protein), binds ATP, opioids and phosphatidylethanolamine. PBP is able to regulate Aurora kinase B expression and the spindle checkpoint through interaction with the RAF1/MEK/ERK cascade. PBP is able to bind to RAF1 and MEK and competitively disrupt the interaction between these proteins. PBP may be involved in the function of the presynaptic cholinergic neurons of the central nervous system. It increases the production of choline acetyltransferase but not acetylcholinesterase. PBP has been implicated as a suppressor of metastasis.Synonyms: HCNPpp, PBP, PEBP, PEBP-1, Phosphatidylethanolamine-binding protein 1, Prostatic-binding protein, Raf kinase inhibitor protein

Gen-ID: 5037

NCBI Accession: NP_002558

UniProt: P30086

Pathways: Feeding Behaviour