NQO1 Antikörper (C-Term)

Produktnummer ABIN374187

Zitiert in 2+ Publikationen. Der Ziege Polyklonal anti-NQO1 Antikörper (ABIN374187) detektiert spezifisch NQO1 in WB und EIA. Dieser Antikörper reagiert spezifisch mit Proben aus Human, Maus, Ratte, Hund und Schwein.

Kurzübersicht für NQO1 Antikörper (C-Term) (ABIN374187)

Target: NQO1 (NAD(P)H Dehydrogenase, Quinone 1 (NQO1))

Reaktivität: Human, Maus, Ratte, Hund, Schwein

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser NQO1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Enzyme Immunoassay (EIA)

Produktdetails anti-NQO1 Antikörper

Bindungsspezifität: C-Term

Sequenz: C-SIPTDNQIKA RK

Spezifität: This antibody is expected to recognize all three reported isoforms (NP_000894.1, NP_001020604.1 and NP_001020605.1) of NQO1.

Kreuzreaktivität (Details): Species reactivity (expected):Rat, Pig.
Species reactivity (tested):Human, Mouse, Canine.

Aufreinigung: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide

Immunogen: Peptide from C Terminus of the protein sequence according to NP_000894.1, NP_001020604.1, NP_001020605.1. Genename: NQO1

Anwendungsinformationen

Applikationshinweise: Peptide ELISA: Limit dilution 1: 64000. Western blot: 0.03-0.1 μg/mL. Approx.30 kDa band observed in Human Kidney and Lunglysates (calculated MW of 30.0 kDa according to NP_000894.1).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Referenzen für anti-NQO1 Antikörper (ABIN374187)

Tanaka, Aleksunes, Cui, Klaassen: "ANIT-induced intrahepatic cholestasis alters hepatobiliary transporter expression via Nrf2-dependent and independent signaling." in: Toxicological sciences : an official journal of the Society of Toxicology, Vol. 108, Issue 2, pp. 247-57, (2009) (PubMed).

Vincent, Kato, McLean, Soules, Feldman: "Sensory neurons and schwann cells respond to oxidative stress by increasing antioxidant defense mechanisms." in: Antioxidants & redox signaling, Vol. 11, Issue 3, pp. 425-38, (2009) (PubMed).

Details zu NQO1

Target: NQO1 (NAD(P)H Dehydrogenase, Quinone 1 (NQO1))

Andere Bezeichnung: NQO1

Hintergrund: NQO1 is a member of the NAD(P)H dehydrogenase (quinone) family and encodes a cytoplasmic 2-electron reductase. This FAD-binding protein forms homodimers and reduces quinones to hydroquinones. NQO1's enzymatic activity prevents the one electron reduction of quinones that results in the production of radical species. NQO1 functions as an important part of cellular antioxidant defense by detoxifying quinones thus preventing the formation of reactive oxygen species. Mutations in the gene for NQO1 have been associated with tardive dyskinesia (TD), an increased risk of hematotoxicity after exposure to benzene, and susceptibility to various forms of cancer. Altered expression of NQO1 has been seen in many tumors and is also associated with Alzheimer's disease (AD). NQO1 levels have been shown to be raised in may tumour types. This has led to a search for anti-tumour agents that can be activated by NQO1 resulting in specific targetting of these compounds to the tumour site. Many cytotoxic drugs contain the quinone moiety, such as the benzoquinone group, which can be activated by NQO1 through the reduction into hydroquinone [which in this case can auto-oxidize and lead to the formation of free radicals and alkylating species]. However, the rat enzyme has been shown to be significantly more effective in activating some of these compounds than the human and mouse enzymes. This indicates that the available cytotoxic drugs are better substrates for the rat enzyme and are not the most ideal prodrugs for activation by NQO1 in human tumors.Synonyms: Azoreductase, DIA4, DT-diaphorase, Menadione reductase, NAD(P)H dehydrogenase [quinone] 1, NAD(P)H:quinone oxidoreductase 1, NMOR1, Phylloquinone reductase, Quinone reductase 1

Gen-ID: 1728

NCBI Accession: NP_000894

UniProt: P15559