Verified
ALS2
Reaktivität: Human
ELISA, IHC
Wirt: Ziege
Polyclonal
unconjugated
Applikationshinweise
Peptide ELISA: Limit dilution 1: 8000. Immunohistochemistry: 3-5 μg/mL. In paraffin embedded Human Cortex shows pixulatecytoplasm staining in some neuronal cells. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Konzentration
0.5 mg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung
Avoid repeated freezing and thawing.
Lagerung
4 °C/-20 °C
Informationen zur Lagerung
Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C.
ALS2 specifically binds to, and functions as a guanine nucleotide exchange factor (GEF) for the small GTPase RAB5. ALS2 contains an ATS1/RCC1-like domain, a RhoGEF domain, and a vacuolar protein sorting 9 (VPS9) domain, all of which are guanine-nucleotide exchange factors that activate members of the Ras superfamily of GTPases. The protein localizes with RAB5 on early endosomal compartments, and functions as a modulator for endosomal dynamics. Loss of ALS2 function might underlie neuronal dysfunction and degeneration in a number of motor neuron diseases.Synonyms: ALS2CR6, Amyotrophic lateral sclerosis 2 chromosomal region candidate gene 6 protein, Amyotrophic lateral sclerosis protein 2, KIAA1563