FOXP3 Antikörper (C-Term)

Produktnummer ABIN374156

Der Ziege Polyklonal Anti-FOXP3-Antikörper wurde für WB und EIA validiert. Er ist geeignet, FOXP3 in Proben von Human, Schwein und Hund zu detektieren.

Kurzübersicht für FOXP3 Antikörper (C-Term) (ABIN374156)

Target: FOXP3 (Forkhead Box P3 (FOXP3))

Reaktivität: Human, Schwein, Hund

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser FOXP3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Enzyme Immunoassay (EIA)

Produktdetails anti-FOXP3 Antikörper

Bindungsspezifität: C-Term

Sequenz: SQRPSRCSNP TPGP

Spezifität: This antibody is expected to recognize both reported isoforms (NP_054728.2 and NP_001107849.1).

Kreuzreaktivität (Details): Species reactivity (expected):Canine (Dog) and Pig.
Species reactivity (tested):Human.

Aufreinigung: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide

Immunogen: Peptide from C-Terminus of the protein sequence according to NP_054728.2, NP_001107849.1. Genename: FOXP3

Anwendungsinformationen

Applikationshinweise: Peptide ELISA: Limit Dilution: 1/16000. Immunohistochemistry: 0.3-1 μg/mL. In paraffin embedded Human Tonsil, stains nuclei ofsome cells in the interfollicular area.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu FOXP3

Target: FOXP3 (Forkhead Box P3 (FOXP3))

Andere Bezeichnung: FOXP3

Hintergrund: FOXP3 (a 431 amino acid protein) is a member of the forkhead/winged-helix family of transcriptional regulators and is highly conserved across mammals. FOXP3 is essential for normal immune homeostasis. FOXP3 is stably and constitutively expressed at a high level in CD25 + CD4 positive regulatory T cells, at low level in CD4 positive/CD25 negative cells, and is absent in CD4 negative/CD8 positive T cells. FOXP3 may be a master regulatory gene and a more specific marker of regulatory T cells than other T cells.Synonyms: Forkhead box protein P3, IPEX, JM2, Scurfin

Gen-ID: 50943

NCBI Accession: NP_054728

UniProt: Q9BZS1

Pathways: Chromatin Binding, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Activated T Cell Proliferation