DDAH1 Antikörper (C-Term)

Produktnummer ABIN374149

Dieser Ziege Polyklonal Antikörper detektiert spezifisch DDAH1 in WB. Es zeigt Reaktivität gegenüber Proben von Human und Rind (Kuh).

Kurzübersicht für DDAH1 Antikörper (C-Term) (ABIN374149)

Target: DDAH1 (Dimethylarginine Dimethylaminohydrolase 1 (DDAH1))

Reaktivität: Human, Rind (Kuh)

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser DDAH1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Produktdetails anti-DDAH1 Antikörper

Bindungsspezifität: C-Term

Sequenz: TCCSVLINKK VDS

Spezifität: This antibody is expected to recognize both reported isoforms of DDAH1 (NP_036269.1and NP_001127917.1).

Kreuzreaktivität (Details): Species reactivity (expected):Bovine.
Species reactivity (tested):Human.

Aufreinigung: Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide

Immunogen: Peptide from C-Terminus of the protein sequence according to NP_036269.1, NP_001127917.1 Genename: DDAH1

Anwendungsinformationen

Applikationshinweise: Peptide ELISA: Limit dilution 1: 16000. Western blot: 0.1-0.3 μg/mL. Approx 37 kDa band observed in human kidney, liver andpancreas lysates (calculated MW of 31.7 kDa according to NP_036269.1). The observedmolecular weight corresponds to earlier findings in literature with different antibodies(Pullamsetti et al, FASEB J.2005 Jul,19(9): 1175-7, PMID: 15827267).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu DDAH1

Target: DDAH1 (Dimethylarginine Dimethylaminohydrolase 1 (DDAH1))

Andere Bezeichnung: DDAH1

Hintergrund: DDAH1 belongs to the dimethylarginine dimethylaminohydrolase (DDAH) family. This enzyme plays a role in nitric oxide generation by regulating cellular concentrations of methylarginines, which in turn inhibit nitric oxide synthase activity.This gene belongs to the dimethylarginine dimethylaminohydrolase (DDAH) gene family. The encoded enzyme plays a role in nitric oxide generation by regulating cellular concentrations of methylarginines, which in turn inhibit nitric oxide synthase activity.Synonyms: DDAH, DDAH-1, DDAHI, Dimethylargininase-1, Dimethylarginine dimethylaminohydrolase 1, N(G), N(G)-dimethylarginine dimethylaminohydrolase 1

Gen-ID: 23576

NCBI Accession: NP_036269

UniProt: O94760