CSK Antikörper (C-Term)

Produktnummer ABIN374096

Der Ziege Polyklonal anti-CSK Antikörper wird verwendet zum Nachweis von CSK in Proben von Human, Maus, Ratte, Rind (Kuh) und Hund. Er wurde validiert für WB, IHC (p) und EIA.

Kurzübersicht für CSK Antikörper (C-Term) (ABIN374096)

Target: CSK (C-Src tyrosine Kinase (CSK))

Reaktivität: Human, Maus, Ratte, Rind (Kuh), Hund

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser CSK Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Produktdetails anti-CSK Antikörper

Bindungsspezifität: C-Term

Sequenz: C-EQLEHIKTHE LHL

Spezifität: This antibody detects Tyrosine-Protein Kinase CSK at C-term.

Kreuzreaktivität (Details): Species reactivity (expected):Canine (Dog), Cow.
Species reactivity (tested):Human, Mouse and Rat.

Aufreinigung: Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide.

Immunogen: Peptide from the C Terminus of the protein sequence according to NP_004374.1, NP_001120662.1 Genename: 1445

Anwendungsinformationen

Applikationshinweise: Peptide ELISA: Limit dilution 1: 32000. Western blot: 0.5-2 μg/mL. Approx 50 kDa band observed in Jurkat lysate (predicted MW of50.0 kDa according to NP_004374.1). Immunohistochemistry: 0.1-0.3 μg/mL. In paraffin embedded human tonsil, strong stainingof germinal centre cells and less intense labelling in the interfollicular area.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu CSK

Target: CSK (C-Src tyrosine Kinase (CSK))

Andere Bezeichnung: CSK

Hintergrund: C terminal Src kinase (CSK) is a non receptor protein tyrosine kinase. One of its major functions is to specifically phosphorylate a conserved C terminal tyrosine of Src family kinases thereby down regulating their kinase activity. CSK is expressed ubiquitously as a protein of approximately 50 KDa in cytosol, but a fraction distributes to lipid rafts due to interaction with the lipid raft associated protein PAG (CSK binding protein).Synonyms: C-SRC kinase, Protein-tyrosine kinase CYL, Tyrosine-protein kinase CSK

Gen-ID: 1445

NCBI Accession: NP_004374

UniProt: P41240

Pathways: T-Zell Rezeptor Signalweg, EGFR Signaling Pathway, Cell-Cell Junction Organization, CXCR4-mediated Signaling Events