ALDH1A1 Antikörper (C-Term)

Produktnummer ABIN374078

Es gibt 1 Publikation für dieses Produkt. Der Ziege Polyklonal anti-ALDH1A1 Antikörper wird verwendet zum Nachweis von ALDH1A1 in Proben von Human, Maus, Ratte, Rind (Kuh) und Hund. Er wurde validiert für WB, IHC (p) und EIA.

Kurzübersicht für ALDH1A1 Antikörper (C-Term) (ABIN374078)

Target: ALDH1A1 (Aldehyde Dehydrogenase 1 Family, Member A1 (ALDH1A1))

Reaktivität: Human, Maus, Ratte, Rind (Kuh), Hund

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser ALDH1A1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Produktdetails anti-ALDH1A1 Antikörper

Bindungsspezifität: C-Term

Sequenz: C-EVKTVTVKIS QKNS

Spezifität: This antibody detects ALDH1 at C-term.

Kreuzreaktivität (Details): Species reactivity (expected):Canine (Dog) and Cow.
Species reactivity (tested):Human, Mouse and Rat.

Aufreinigung: Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide

Immunogen: Peptide from the C-Terminus of the protein according to NP_000680.2. Genename: ALDH1A1

Anwendungsinformationen

Applikationshinweise: Peptide ELISA: Limit dilution 1: 16000. Western blot: 0.03-0.1 μg/mL. Approx 55 kDa band observed in human liver lysates(calculated MW of 54.5 kDa according to NP_000680.2).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3 with 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Referenzen für anti-ALDH1A1 Antikörper (ABIN374078)

Amann, Hofmann, Freudenberger, Holland-Cunz, Eichmüller, Bazhin: "Expression and activity of alcohol and aldehyde dehydrogenases in melanoma cells and in melanocytes." in: Journal of cellular biochemistry, Vol. 113, Issue 3, pp. 792-9, (2012) (PubMed).

Details zu ALDH1A1

Target: ALDH1A1 (Aldehyde Dehydrogenase 1 Family, Member A1 (ALDH1A1))

Andere Bezeichnung: ALDH1

Hintergrund: ALDH1A1 is an aldehyde dehydrogenase able to oxidize a wide variety of aliphatic aldehydes (retinaldehyde, acetaldehyde, etc.) to the corresponding carboxylic acids (retinoic acid, acetic acid, etc.). ALDH1A1 (also known as RALDH1, ALDH1, or AHD2) is highly expressed in the dorsal retina, ventral midbrain (dopaminergic neurons), and hematopoietic stem cells. ALDH1A1 is involved in retinoic acid (RA) synthesis during vertebrate embryogenesis. ALDH1A1 is first detected at E9.0-E10.5 in cranial tissues (ventral mesencephalon, dorsal retina, thymic primordia, optic vesicles) and in the mesonephros. ALDH1A1 is also of interest in Parkinson's Disease (PD) being expressed in the the A9 dopaminergic (DA) neuronal group projecting to the dorsal striatum, this is the most vulnerable site in PD (Chung et al, 2005). Some studies also identify ALDH1A1 as a marker of cancer stem cells. Studies of breast cancer tissues showed that those that expressed a form of ALDH1A1 had the worst outcomes.Synonyms: ALDC, ALDH-E1, ALDH1A1, ALHDII, Aldehyde dehydrogenase cytosolic, Aldehyde dehydrogenase family 1 member A1, PUMB1, RALDH1, Retinal dehydrogenase 1

Gen-ID: 216

NCBI Accession: NP_000680

UniProt: P00352

Pathways: Dopaminergic Neurogenesis