PC Antikörper (Internal Region)

Produktnummer ABIN372799

Dieser Ziege Polyklonal Antikörper detektiert spezifisch PC in WB, IHC (p) und EIA. Es zeigt Reaktivität gegenüber Proben von Human, Maus, Ratte, Schwein, Rind (Kuh) und Hund.

Kurzübersicht für PC Antikörper (Internal Region) (ABIN372799)

Target: PC (Pyruvate Carboxylase (PC))

Reaktivität: Human, Maus, Ratte, Schwein, Rind (Kuh), Hund

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser PC Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Produktdetails anti-PC Antikörper

Bindungsspezifität: Internal Region

Spezifität: Recognizes Pyruvate Carboxylase (PC).

Kreuzreaktivität (Details): Species reactivity (expected):Bovine, Canine (Dog), Mouse, Porcine and Rat.
Species reactivity (tested):Human.

Aufreinigung: Immunoaffinity Chromatography.

Immunogen: Synthetic peptide (C-KFKEVKKAYVEANQ) from an Internal region of Human PC.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: ELISA: 1/16000. Western blot: 0.03-0.1 μg/mL. Immunohistochemistry on Paraffin Sections: 5 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: Tris saline buffer, pH 7.3 containing 0.5 % BSA as stabilizer and 0.02 % Sodium Azide as preservative.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Details zu PC

Target: PC (Pyruvate Carboxylase (PC))

Andere Bezeichnung: Pyruvic Carboxylase (PC)

Hintergrund: Pyruvate Carboxylase (PC, PCB) requires biotin and ATP to catalyse the carboxylation of pyruvate to oxaloacetate. The active enzyme is a homotetramer arranged in a tetrahedron which is located exclusively in the mitochondrial matrix. PCB is involved in gluconeogenesis, lipogenesis, insulin secretion and synthesis of the neurotransmitter glutamate. Mutations in this gene have been associated with pyruvate carboxylase deficiency. Alternatively spliced transcript variants with different 5' UTRs, but encoding the same protein, have been found for this gene.Synonyms: PCB, Pyruvate carboxylase mitochondrial

Gen-ID: 5091

NCBI Accession: NP_000911

UniProt: P11498