HPSE Antikörper

Produktnummer ABIN372789

Der Kaninchen Polyklonal anti-HPSE Antikörper (ABIN372789) detektiert spezifisch HPSE in WB und IHC (p). Dieser Antikörper reagiert spezifisch mit Proben aus Human und Maus.

Kurzübersicht für HPSE Antikörper (ABIN372789)

Target: HPSE (Heparanase (HPSE))

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser HPSE Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-HPSE Antikörper

Spezifität: Recognizes the 65 kDa precursor as well as the 55 kDa and 5 kDa subunits of Human or Mouse Heparanase.

Kreuzreaktivität (Details): Species reactivity (expected):Mouse.
Species reactivity (tested):Human.

Aufreinigung: Protein G Chromatography.

Immunogen: Human 50kD-8kD Heparanase Heterodimer.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western Blot: 1/500. Immunohistochemistry on Paraffin Sections: 5 μg/mL. This HPSE antibody was validated for use in immunohistochemistry on a panel of 21formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigenretrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides wereincubated with biotinylated secondary antibody Rabbit IgG, followed by alkalinephosphatase-streptavidin and chromogen.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: PBS, pH 7.2 containing 0.001 % Thimerosal as preservative.

Konservierungsmittel: Thimerosal (Merthiolate)

Vorsichtsmaßnahmen: This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Details zu HPSE

Target: HPSE (Heparanase (HPSE))

Andere Bezeichnung: Heparanase / HPSE

Hintergrund: Heparanase is an endo-beta-D-glucuronidase, which degrades heparan sulfate side chains of heparan sulfate proteoglycans (HSPGs) in the extracellular matrix. Heparanase plays an important role in ECM degradation, facilitating the migration and extravasation of tumor cells and inflammatory leukocytes. Upon degradation, heparanase releases growth factors and cytokines that stimulate cell proliferation and chemotaxis. Heparanase is a heterodimer comprised of a 50kD subunit harboring the active site and a 8kD subunit. It is produced as a latent 65kD precursor and proteolytically processed to its active form. Heparanase is highly expressed in myeloid leukocytes (i.e. neutrophils) in platelets and in human placenta. Human heparanase was found to be upregulated in various types of primary tumors, correlating in some cases with increased tumor invasiveness and vascularity and with poor prospective survival.Synonyms: Endo-Glucoronidase, HEP, HPA, HPA1, HPR1, HPSE1, HSE1, Heparanase-1

Gen-ID: 10855

NCBI Accession: NP_001092010

UniProt: Q9Y251

Pathways: Glycosaminoglycan Metabolic Process