Tyrosine Hydroxylase Antikörper

Produktnummer ABIN372735

Dieses Anti-Tyrosine Hydroxylase-Antikörper ist ein Schaf Polyklonal-Antikörper zur Detektion von Tyrosine Hydroxylase in WB, IF und IHC (fro). Geeignet für Säugetier.

Kurzübersicht für Tyrosine Hydroxylase Antikörper (ABIN372735)

Target: Tyrosine Hydroxylase (TH)

Reaktivität: Säugetier

Wirt: Schaf

Klonalität: Polyklonal

Konjugat: Dieser Tyrosine Hydroxylase Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro))

Produktdetails anti-Tyrosine Hydroxylase Antikörper

Spezifität: Specific for the ~60k Tyrosine Hydroxylase protein in Western Blots of Rat brain lysates and PC-12 cells stimulated by Okadaic Acid. The antibody cross reacts with all mammalian and at least some non-mammalian forms of the enzyme in Western blots and in IHC/IF.

Kreuzreaktivität (Details): Species reactivity (tested):Mammalia.

Aufreinigung: Affinity Chromatography.

Immunogen: Native rat tyrosine hydroxylase, purified from pheochromocytoma.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western Blot/Dot Blot: 1/1,000. Immunofluorescence 1/1000 (Frozen Sections). Immunohistochemistry on Frozen Sections: 1/1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: 10 mM HEPES ( pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50 % Glycerol.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store the antibody undiluted (in aliquots) at-20 °C.

Details zu Tyrosine Hydroxylase

Target: Tyrosine Hydroxylase (TH)

Andere Bezeichnung: Tyrosine 3-Monooxygenase (TH)

Hintergrund: Tyrosine hydroxylase (TH) is the rate-limiting enzyme in the synthesis of the catecholamines dopamine and norepinephrine. TH antibodies can therefore be used as markers for dopaminergic and noradrenergic neurons in a variety of applications including depression, schizophrenia, Parkinson's disease and drug abuse (Kish et al., 2001, Zhu et al., 2000, Zhu et al., 1999). TH antibodies can also be used to explore basic mechanisms of dopamine and norepinephrine signaling (Witkovsky et al., 2000, Salvatore et al., 2001).Synonyms: TYH, Tyrosine 3-hydroxylase

Gen-ID: 25085

NCBI Accession: NP_036872

UniProt: P04177

Pathways: Dopaminergic Neurogenesis, Response to Water Deprivation, Sensory Perception of Sound, Carbohydrate Homeostasis, Feeding Behaviour