Tyrosine Hydroxylase Antikörper

Produktnummer ABIN372734

Produktdetails anti-Tyrosine Hydroxylase Antikörper

Target: Tyrosine Hydroxylase (TH)

Reaktivität: Säugetier

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Tyrosine Hydroxylase Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro)), Dot Blot (DB)

Spezifität: Specific for the ~60k tyrosine hydroxylase protein. The antibody recognizes all mammalian and at least some non-mammalian forms of the enzyme in Western blots and in Immunohistochemistry/Immunofluorescence.

Kreuzreaktivität (Details): Species reactivity (tested):Mammalia.

Aufreinigung: Affinity Chromatography on Protein A.

Immunogen: SDS-denatured rat tyrosine hydroxylase, purified from pheochromocytoma.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blotting: 1/1,000. Immunofluorescence 1/1000 (Frozen Sections). Immunohistochemistry on Frozen Sections: 1/1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: 10 mM HEPES ( pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50 % Glycerol.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: -20 °C/-80 °C

Informationen zur Lagerung: Store the antibody undiluted (in aliquots) at-20 °C or (in aliquots) at -80 °C for long term storage.

Referenzen für anti-Tyrosine Hydroxylase Antikörper (ABIN372734)

Grapensparr, Vasylovska, Li, Olerud, Jansson, Kozlova, Carlsson: "Co-transplantation of human pancreatic islets with post-migratory neural crest stem cells increases β-cell proliferation and vascular and neural regrowth." in: The Journal of clinical endocrinology and metabolism, Vol. 100, Issue 4, pp. E583-90, (2015) (PubMed).

Kirane, Toombs, Larsen, Ostapoff, Meshaw, Zaknoen, Brekken, Burrows: "Epithelial-mesenchymal transition increases tumor sensitivity to COX-2 inhibition by apricoxib." in: Carcinogenesis, Vol. 33, Issue 9, pp. 1639-46, (2012) (PubMed).

Kish, Kalasinsky, Derkach, Schmunk, Guttman, Ang, Adams, Furukawa, Haycock: "Striatal dopaminergic and serotonergic markers in human heroin users." in: Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology, Vol. 24, Issue 5, pp. 561-7, (2001) (PubMed).

Salvatore, Waymire, Haycock: "Depolarization-stimulated catecholamine biosynthesis: involvement of protein kinases and tyrosine hydroxylase phosphorylation sites in situ." in: Journal of neurochemistry, Vol. 79, Issue 2, pp. 349-60, (2001) (PubMed).

Witkovsky, Gabriel, Haycock, Meller: "Influence of light and neural circuitry on tyrosine hydroxylase phosphorylation in the rat retina." in: Journal of chemical neuroanatomy, Vol. 19, Issue 2, pp. 105-16, (2000) (PubMed).

Zhu, Klimek, Haycock, Ordway: "Quantitation of tyrosine hydroxylase protein in the locus coeruleus from postmortem human brain." in: Journal of neuroscience methods, Vol. 99, Issue 1-2, pp. 37-44, (2000) (PubMed).

Details zu Tyrosine Hydroxylase

Target: Tyrosine Hydroxylase (TH)

Andere Bezeichnung: Tyrosine 3-Monooxygenase (TH) (TH Produkte)

Synonyme: DYT14 antikoerper, DYT5b antikoerper, TYH antikoerper, The antikoerper, CG10118 antikoerper, DH65B antikoerper, DTH antikoerper, Dmel\\CG10118 antikoerper, Pale antikoerper, Ple antikoerper, TH antikoerper, Th antikoerper, VII antikoerper, dTH1 antikoerper, dTH65B antikoerper, pale/ple antikoerper, th antikoerper, dyt14 antikoerper, dyt5b antikoerper, tyh antikoerper, tyrosine hydroxylase antikoerper, pale antikoerper, tyrosine 3-monooxygenase antikoerper, tyrosine hydroxylase S homeolog antikoerper, TH antikoerper, Th antikoerper, ple antikoerper, th antikoerper, th.S antikoerper, LOC100008895 antikoerper

Hintergrund: Tyrosine hydroxylase (TH) is the rate-limiting enzyme in the synthesis of the catecholamines dopamine and norepinephrine. TH antibodies can therefore be used as markers for dopaminergic and noradrenergic neurons in a variety of applications including depression, schizophrenia, Parkinson's disease and drug abuse (Kish et al., 2001, Zhu et al., 2000, Zhu et al., 1999). TH antibodies can also be used to explore basic mechanisms of dopamine and norepinephrine signaling (Witkovsky et al., 2000, Salvatore et al., 2001, Dunkley et al., 2004).Synonyms: TYH, Tyrosine 3-hydroxylase

Gen-ID: 25085

NCBI Accession: NP_036872

UniProt: P04177

Pathways: Dopaminergic Neurogenesis, Response to Water Deprivation, Sensory Perception of Sound, Carbohydrate Homeostasis, Feeding Behaviour