SYT1 Antikörper (pThr202)

Produktnummer ABIN372724

Dieser Kaninchen Polyklonal Antikörper detektiert spezifisch SYT1 in WB, IHC (fro) und DB. Es zeigt Reaktivität gegenüber Proben von Human, Ratte, Maus, Rind (Kuh), Huhn, Hund, Zebrafisch (Danio rerio) und Non-Human Primate.

Kurzübersicht für SYT1 Antikörper (pThr202) (ABIN372724)

Target: SYT1 (Synaptotagmin I (SYT1))

Reaktivität: Human, Ratte, Maus, Rind (Kuh), Huhn, Hund, Zebrafisch (Danio rerio), Non-Human Primate

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser SYT1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Dot Blot (DB)

Produktdetails anti-SYT1 Antikörper

Bindungsspezifität: pThr202

Spezifität: This antibody recognizes the ~60k-62k synaptotagmin protein phosphorylated at Thr202 in Western Blots of Rat brain lysate. The immunolabeling is completely eliminated by lambda-phosphatase treatment.

Kreuzreaktivität (Details): Species reactivity (expected):Human, Bovine, Canine, Chicken, Mouse, non-Human Primate and Zebrafish.
Species reactivity (tested):Rat.

Aufreinigung: Sequential Chromatography on phospho- and dephosphopeptide affinity columns.

Immunogen: Phosphopeptide corresponding to amino acid residues surrounding the phospho-Thr202 of Synaptotagmin.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Dot blot: 1/1,000. Western blot: 1/1,000. Immunohistochemistry: 1/400.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: 10 mM HEPES ( pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50 % Glycerol.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store the antibody undiluted (in aliquots) at-20 °C.

Details zu SYT1

Target: SYT1 (Synaptotagmin I (SYT1))

Andere Bezeichnung: Synaptotagmin-1

Hintergrund: Synaptotagmin is widely regarded as the primary calcium sensor for synaptic vesicle exocytosis (Fernandez-Chacon et al., 2001, Wang et al., 2003). Moreover, recent studies indicate that the protein also plays a key role in endocytosis (Poskanzer et al., 2003). Synaptotagmin can be phosphorylated by multiple protein kinases and this may play a key role in modulation of synaptotagmin's ability to influence both the exocytotic and endocytotic components of synaptic transmission (Hilfiker et al., 1999, Lee et al., 2004).Synonyms: SYT1, SYT2, SYT3, SYT4, SYT5

Gen-ID: 6857

NCBI Accession: NP_001129277

UniProt: P21579

Pathways: Synaptic Vesicle Exocytosis, Dicarboxylic Acid Transport