SYN1 Antikörper (pSer9)

Produktnummer ABIN372718

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch SYN1 in WB und IHC (fro). Er zeigt eine Reaktivität gegenüber Human, Ratte, Maus, Rind (Kuh), Xenopus laevis und Zebrafisch (Danio rerio).

Kurzübersicht für SYN1 Antikörper (pSer9) (ABIN372718)

Target: SYN1 (Synapsin I (SYN1))

Reaktivität: Human, Ratte, Maus, Rind (Kuh), Xenopus laevis, Zebrafisch (Danio rerio)

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser SYN1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro))

Produktdetails anti-SYN1 Antikörper

Bindungsspezifität: pSer9

Spezifität: This antibody recognizes the ~78k Synapsin I doublet protein phosphorylated at Ser9. The antibody also weakly labels the ~55k Synapsin II protein which has a similar phosphorylation site to that of Ser9 on Synapsin I. Immunolabeling is blocked by preadsorption of the antibody with the phosphopeptide used as antigen but not by the corresponding dephosphopeptide. Immunolabeling is also completely eliminated by treatment with lambda-phosphatase.

Kreuzreaktivität (Details): Species reactivity (expected):Human, Bovine, Mouse, Xenopus and Zebrafish.
Species reactivity (tested):Rat.

Aufreinigung: Sequential Chromatography on phospho- and dephosphopeptide affinity columns.

Immunogen: Phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser9 of Synapsin I.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot: 1/1,000. Immunohistochemistry: 1/500.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: 10 mM HEPES ( pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50 % Glycerol.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store the antibody undiluted (in aliquots) at-20 °C.

Details zu SYN1

Target: SYN1 (Synapsin I (SYN1))

Andere Bezeichnung: Synapsin-1

Hintergrund: Synapsin I plays a key role in synaptic plasticity in brain (Feng et al., 2002, Nayak et al., 1996). This effect is due in large part to the ability of the synapsins to regulate the availability of synaptic vesicles for release. In addition to its role in plasticity, the expression of synapsin I is a precise indicator of synapse formation (Moore and Bernstein, 1989, Stone et al., 1994). Thus, synapsin I immunocytochemistry provides a valuable tool for the study of synaptogenesis. The role of synapsin in synaptic plasticity and in synaptogensis is regulated by phosphorylation (Jovanovic et al., 2001, Kao et al., 2002). Serine 9 is the site on synapsin I that is phosphorylated by cAMP-dependent protein kinase and by calcium calmodulin kinase I (Czernik et al., 1987). Phosphorylation of this site is thought to regulate synaptic vesicle function and neurite outgrowth (Kao et al., 2002).Synonyms: Brain protein 4.1, SYN1, Synapsin I

Gen-ID: 6853

NCBI Accession: NP_008881

UniProt: P17600