Periostin Antikörper (C-Term)

Produktnummer ABIN372700

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch Periostin in WB und IHC (p). Er zeigt eine Reaktivität gegenüber Human, Maus, Ratte, Huhn und Säugetier.

Kurzübersicht für Periostin Antikörper (C-Term) (ABIN372700)

Target: Periostin (POSTN)

Reaktivität: Human, Maus, Ratte, Huhn, Säugetier

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Periostin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-Periostin Antikörper

Bindungsspezifität: C-Term

Spezifität: This antibody recognizes the ~93 kDa Periostin doublet in mouse lung extract. This antibody does not recognize the single heart form of Periostin or the lowest molecular weight form of lung periostin. Consistent with the fact that this antibody is made against an alternatively spliced region of periostin, this antibody recognizes a subset of the forms of Periostin recognized on Western blots by the Pan Periostin antibody (Cat#AP08724AF-N) and also shows a distinctive staining pattern by Immunohistochemistry (data not shown).

Kreuzreaktivität (Details): Species reactivity (expected):Mammalia and Avian.
Species reactivity (tested):Human, Mouse, Rat and Chicken.

Aufreinigung: Affinity Chromatography.

Immunogen: Bacterial fusion protein equivalent to a 188-amino acid polypeptide from the C-terminal region of mouse Periostin which is comprised of six small alternatively-spliced exons.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot: 1/1000. Immunohistochemistry: 1/100. The antibody works well for Immunohistochemistry on paraformaldehyde-fixed sectionswith a simple antigen-retrieval protocol (incubate slides for 20 minutes at 90 °C in 10 mMsodium citrate ( pH 6.0)/ 0.1 % Tween-20).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: PBS without preservatives.

Konservierungsmittel: Without preservative

Handhabung: Avoid repeated freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store the antibody undiluted (in aliquots) at-20 °C.

Details zu Periostin

Target: Periostin (POSTN)

Andere Bezeichnung: Periostin

Hintergrund: Periostin is a matricellular protein, i.e. an extracellular matrix protein that interacts both with other ECM proteins and with cell-surface receptors. Like many other matricellular proteins, the function of periostin is important both in embryonic development and in the remodeling of adult tissues in response to pathological insults. Periostin was originally isolated as an osteoblast-specific marker that functions as a cell adhesion molecule for preosteoblasts and is thought to be involved in osteoblast recruitment, attachment and spreading (Kruzynska-Frejtag A. et al., 2004). Periostin has recently been shown to promote collagen fibrogenesis, inhibit differentiation of progenitor cells into cardiomyocytes and to be essential in maintaining the biomechanical properties of the adult myocardium (Norris et al., 2008)Synonyms: OSF-2, OSF2, Osteoblast-specific factor 2, PN, POSTN

Gen-ID: 10631

NCBI Accession: NP_001129406

UniProt: Q15063