Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro))
Spezifität
Specific for the ~50k Gap-43 protein phosphorylated at Ser41 in Western Blots of Rat brain lysate. In some tissues the antibody also recognizes a higher molecular weight protein that is also recognized by the pan Gap-43 antibody, that may be a Gap-43 aggregate or oligomer. Immunolableing is blocked by the phosphopeptide used as antigen but not by the corresponding dephosphopeptide. Immunolabeling is completely eliminated by treatment with lambda-Ptase.
Kreuzreaktivität (Details)
Species reactivity (expected):Bovine, Canine, Chicken, Finch, Human, Mouse, non-Human Primates, Xenopus and Zebrafish. Species reactivity (tested):Rat.
Aufreinigung
Sequential Chromatography on phospho- and dephosphopeptide affinity columns.
Immunogen
Phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser41 of Gap-43.
Western blot: 1/1000. Immunohistochemitsry. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Liquid
Buffer
10 mM HEPES ( pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50 % Glycerol.
Handhabung
Avoid repeated freezing and thawing.
Lagerung
-20 °C
Informationen zur Lagerung
Store the antibody undiluted (in aliquots) at-20 °C.
B-50 antikoerper, PP46 antikoerper, Basp2 antikoerper, GAP-43 antikoerper, b-50 antikoerper, gap-43 antikoerper, gap43b antikoerper, pp46 antikoerper, cb310 antikoerper, gap antikoerper, gap43 antikoerper, gap43a antikoerper, growth associated protein 43 antikoerper, growth associated protein 43 L homeolog antikoerper, growth associated protein 43 S homeolog antikoerper, GAP43 antikoerper, Gap43 antikoerper, gap43.L antikoerper, gap43 antikoerper, gap43.S antikoerper
Hintergrund
Gap-43 is thought to have an important role in development and plasticity because it is expressed at high levels in neuronal growth cones during development and during axonal regeneration (Benowitz and Routtenberg, 1997). There is also evidence from knockout animals that Gap-43 serves to amplify pathfinding signals from the growth cone (Strittmatter et al., 1995). Gap-43 is thought to mediate at least some of these effects via interaction with actin. Importantly, phosphorylation at Ser41 by protein kinase C (Catalog No. 1609-PKC) modulates the interaction of Gap-43 with actin (He et al., 1997) and may also affect neurotransmitter release during forms of plasticity like LTP (Hulo et al., 2002).Synonyms: Axonal membrane protein GAP-43, Growth-associated protein 43, Neural phosphoprotein B-50, Neuromodulin, PP46