Dynamin 1 Antikörper (pSer774)
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- Target Alle Dynamin 1 (DNM1) Antikörper anzeigen
- Dynamin 1 (DNM1)
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Bindungsspezifität
- pSer774
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Reaktivität
- Human, Maus, Ratte, Hund, Rind (Kuh), Huhn, Non-Human Primate
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Wirt
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Schaf
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Klonalität
- Polyklonal
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Konjugat
- Dieser Dynamin 1 Antikörper ist unkonjugiert
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Applikation
- Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro))
- Spezifität
- Specific for the ~95k Dynamin protein phosphorylated at Ser774. This antiboy labels the purified protein phosphorylated in vitro by CDK5 but not by PKC. Does not cross react with other purified substrates of CDK5 (e.g. Amphiphysin and Synapsin). Immunolabeling is blocked by lambda-phosphatase treatment.
- Kreuzreaktivität (Details)
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Species reactivity (expected):Human, Bovine, Canine, Chicken, Mouse and non-Human Primates.
Species reactivity (tested):Rat. - Aufreinigung
- Sequential Chromatography on phospho- and dephosphopeptide affinity columns.
- Immunogen
- Phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser774 of rat Dynamin.
- Isotyp
- IgG
- Top Product
- Discover our top product DNM1 Primärantikörper
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- Applikationshinweise
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Western Blot: 1/1000. Immunohistochemistry (Fixed Cultured Neurons): 1/1000. (See Ref.3)Immunofluorescence (Fixed Cultured Neurons): 1/1000. (See Ref.3)
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Liquid
- Buffer
- 10 mM HEPES ( pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50 % Glycerol.
- Handhabung
- Avoid repeated freezing and thawing.
- Lagerung
- -20 °C
- Informationen zur Lagerung
- Store the antibody undiluted (in aliquots) at-20 °C.
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- Target
- Dynamin 1 (DNM1)
- Andere Bezeichnung
- Dynamin-1 (DNM1 Produkte)
- Synonyme
- DNM antikoerper, Dnm antikoerper, AI838169 antikoerper, Ftfl antikoerper, mKIAA4093 antikoerper, dnm antikoerper, DNM1 antikoerper, Dnm1 antikoerper, dnm1 antikoerper, si:dkey-246l19.2 antikoerper, dynamin 1 antikoerper, dynamin-1 antikoerper, dynamin 1a antikoerper, DNM1 antikoerper, Dnm1 antikoerper, BACOIKO008_37 antikoerper, PTRG_07818 antikoerper, PTRG_08097 antikoerper, dnm1 antikoerper, LOC100168088 antikoerper, LOC100199205 antikoerper, dnm1a antikoerper
- Hintergrund
- Dynamin is a member of a group of nerve terminal proteins called dephosphins that regulate synaptic vesicle endocytosis (Cousin et al., 2001, Graham et al., 2002, Tsuboi et al., 2002). Cyclin dependent protein kinase 5 phosphorylates Dynamin at Ser774 and Ser778 that are the phosphorylation sites on Dynamin phosphorylated in vivo (Tan et al., 2003). Phosphorylation of these sites on Dynamin is thought to play a key role in synaptic vesicle trafficking.Synonyms: DNM, DNM1
- Gen-ID
- 140694
- NCBI Accession
- NP_542420
- UniProt
- P21575
- Pathways
- Toll-Like Receptors Cascades, CXCR4-mediated Signaling Events, Thromboxane A2 Receptor Signaling
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