DDC Antikörper

Produktnummer ABIN372608

Der Kaninchen Polyklonal anti-DDC Antikörper wird verwendet zum Nachweis von DDC in Proben von Ratte und Rind (Kuh). Er wurde validiert für WB.

Kurzübersicht für DDC Antikörper (ABIN372608)

Target: DDC (Dopa Decarboxylase (Aromatic L-Amino Acid Decarboxylase) (DDC))

Reaktivität: Ratte, Rind (Kuh)

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser DDC Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Produktdetails anti-DDC Antikörper

Spezifität: Specific for the ~55k DDC protein.

Kreuzreaktivität (Details): Species reactivity (expected):Bovine.
Species reactivity (tested):Rat.

Aufreinigung: Affinity Chromatography using an AminoLink® Plus column matrix to which purified recombinant Bovine DDC was coupled.

Immunogen: SDS denatured, recombinant bovine aromatic DOPA decarboxylase (DDC) expressed in E. coli and purified from inclusion bodies.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western Blot: 1/1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: 10 mM HEPES ( pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50 % Glycerol.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store the antibody undiluted (in aliquots) at-20 °C.

Details zu DDC

Target: DDC (Dopa Decarboxylase (Aromatic L-Amino Acid Decarboxylase) (DDC))

Andere Bezeichnung: AADC / DDC

Hintergrund: DOPA decarboxylase is an enzyme implicated in 2 metabolic pathways, synthesizing 2 important neurotransmitters: dopamine and serotonin which both play key roles in many clinical disorders, including Parkinson's disease. Following the hydroxylation of tyrosine to form L dihydroxyphenylalanine (LDOPA), catalyzed by tyrosine hydroxylase, DDC decarboxylates LDOPA to form dopamine. This neurotransmitter is found in different areas of the brain and is particularly abundant in basal ganglia. Dopamine is also produced by DDC in the sympathetic nervous system and is the precursor of the catecholaminergic hormones, noradrenaline and adrenaline in the adrenal medulla. In the nervous system, tryptophan hydroxylase produces 5 OH tryptophan, which is decarboxylated by DDC, giving rise to serotonin. DDC is a homodimeric, pyridoxal phosphate dependent enzyme.Synonyms: Aromatic-L-amino-acid decarboxylase, DOPA decarboxylase

Gen-ID: 24311

NCBI Accession: NP_036677

UniProt: P14173

Pathways: Dopaminergic Neurogenesis